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Literature DB >> 16661150

Assessment of cytoplasmic contaminations in isolated vacuole preparations.

Abstract

A method for testing the purity of isolated vacuoles is proposed. Preparations of isolated vacuoles often contain "impure vacuoles," namely, vacuoles contaminated with some cytoplasmic remnants. Such impure vacuoles are stained by the vital fluorescent dye fluorescein-diacetate. Vacuoles enclosed only by the tonoplast, "naked vacuoles," do not stain.

Entities: Chemical

Year: 1980 PMID： 16661150 PMCID： PMC440272 DOI： 10.1104/pp.65.1.85

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

2 in total

1. Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.

Authors: B Rotman; B W Papermaster
Journal: Proc Natl Acad Sci U S A Date: 1966-01 Impact factor: 11.205

2. Isolation of Vacuoles from Root Storage Tissue of Beta vulgaris L.

Authors: R A Leigh; D Branton
Journal: Plant Physiol Date: 1976-11 Impact factor: 8.340

2 in total

13 in total

1. Inhibition of na/h antiport activity in sugar beet tonoplast by analogs of amiloride.

Authors: E Blumwald; E J Cragoe; R J Poole
Journal: Plant Physiol Date: 1987-09 Impact factor: 8.340

2. Isolation and Characterization of Vacuoles from Melilotus alba Mesophyll.

Authors: A M Boudet; H Canut; G Alibert
Journal: Plant Physiol Date: 1981-12 Impact factor: 8.340

3. Enzymic and protein character of tonoplast from hippeastrum vacuoles.

Authors: G J Wagner
Journal: Plant Physiol Date: 1981-08 Impact factor: 8.340

4. Visualization of vacuoplasts in isolated vacuole preparations from mesophyll protoplasts of periwinkle [Catharanthus roseus (L.) G. Don].

Authors: D McCaskill; A I Scott
Journal: Plant Cell Rep Date: 1992-06 Impact factor: 4.570

5. Localization of the Enzymes of Fructan Metabolism in Vacuoles Isolated by a Mechanical Method from Tubers of Jerusalem Artichoke (Helianthus tuberosus L.).

Authors: C W Darwen; P John
Journal: Plant Physiol Date: 1989-02 Impact factor: 8.340

Assessment of cytoplasmic contaminations in isolated vacuole preparations.

1. Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.

2. Isolation of Vacuoles from Root Storage Tissue of Beta vulgaris L.

1. Inhibition of na/h antiport activity in sugar beet tonoplast by analogs of amiloride.

2. Isolation and Characterization of Vacuoles from Melilotus alba Mesophyll.

3. Enzymic and protein character of tonoplast from hippeastrum vacuoles.

4. Visualization of vacuoplasts in isolated vacuole preparations from mesophyll protoplasts of periwinkle [Catharanthus roseus (L.) G. Don].

5. Localization of the Enzymes of Fructan Metabolism in Vacuoles Isolated by a Mechanical Method from Tubers of Jerusalem Artichoke (Helianthus tuberosus L.).

6. Vacuolar Localization of Endoproteinases EP(1) and EP(2) in Barley Mesophyll Cells.

7. Proton transport in isolated vacuoles from corn coleoptiles.

8. Identification of the leaf vacuole as a major nitrate storage pool.

9. Purification and functional characterization of protoplasts and intact vacuoles from grape cells.

10. Natural antioxidants protect against cadmium-induced damage during pregnancy and lactation in rats' pups.