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Literature DB >> 16659708

Purification and properties of two proteolytic enzymes with carboxypeptidase activity in germinated wheat.

Abstract

Two proteolytic enzymes with carboxypeptidase activity have been isolated from a germinated wheat extract and partially characterized. Both enzymes rapidly released amino acids from hemoglobin and gluten and hydrolyzed carbobenzoxy-phenylalanylalanine. The enzymes were inhibited by diisopropylphosphofluoridate, but unaffected by salts, ethylenediaminetetraacetate, and sulfhydryl reagents at lower concentrations, and had molecular weights of approximately 55,000 and 61,000. Analysis of the hydrolysis products of hemoglobin and gluten indicated that both enzymes had broad specificities, including the ability to release proline.

Entities: Chemical Species

Year: 1976 PMID： 16659708 PMCID： PMC543262 DOI： 10.1104/pp.58.4.516

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

14 in total

1. PURIFICATION AND PROPERTIES OF A NEW CARBOXYPEPTIDASE FROM CITRUS FRUIT.

Authors: H ZUBER
Journal: Nature Date: 1964-02-08 Impact factor: 49.962

2. The developmental biochemistry of cottonseed embryogenesis and germination. II. Catalytic properties of the cotton carboxypeptidase.

Authors: J N Ihle; L S Dure
Journal: J Biol Chem Date: 1972-08-25 Impact factor: 5.157

3. Purification of wheat proteases by affinity chromatography on hemoglobin-Sepharose column.

Authors: G K Chua; W Bushuk
Journal: Biochem Biophys Res Commun Date: 1969-10-22 Impact factor: 3.575

4. A carboxypeptidase from germinated barley and its action on casein.

Authors: M Moeller; G S Robbins; W C Burger; N Prentice
Journal: J Agric Food Chem Date: 1970 Sep-Oct Impact factor: 5.279

5. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors: K Weber; M Osborn
Journal: J Biol Chem Date: 1969-08-25 Impact factor: 5.157

6. Isolation and properties of protease A from wheat grain.

Authors: J Skupin; J Warchalewski
Journal: J Sci Food Agric Date: 1971-01 Impact factor: 3.638

7. Partial purification and enzymatic properties of an aminopeptidase from barley.

Authors: L Kolehmainen; J Mikola
Journal: Arch Biochem Biophys Date: 1971-08 Impact factor: 4.013

8. Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.

Authors: O Vesterberg; H Svensson
Journal: Acta Chem Scand Date: 1966

9. Purification and properties of a proteolytic enzyme from French beans.

Authors: J R Wells
Journal: Biochem J Date: 1965-10 Impact factor: 3.857

10. Estimation of the molecular weights of proteins by Sephadex gel-filtration.

Authors: P Andrews
Journal: Biochem J Date: 1964-05 Impact factor: 3.766

10 in total

1. Physiological control of exo- and endoproteolytic activities in germinating wheat and their relationship to storage protein hydrolysis.

Authors: K R Preston; J E Kruger
Journal: Plant Physiol Date: 1979-09 Impact factor: 8.340

6. Hemoglobin-digesting Acid Proteinases in Soybean Leaves: CHARACTERISTICS AND CHANGES DURING LEAF MATURATION AND SENESCENCE.

Authors: L V Ragster; M J Chrispeels
Journal: Plant Physiol Date: 1981-01 Impact factor: 8.340

Purification and properties of two proteolytic enzymes with carboxypeptidase activity in germinated wheat.

1. PURIFICATION AND PROPERTIES OF A NEW CARBOXYPEPTIDASE FROM CITRUS FRUIT.

2. The developmental biochemistry of cottonseed embryogenesis and germination. II. Catalytic properties of the cotton carboxypeptidase.

3. Purification of wheat proteases by affinity chromatography on hemoglobin-Sepharose column.

4. A carboxypeptidase from germinated barley and its action on casein.

5. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

6. Isolation and properties of protease A from wheat grain.

7. Partial purification and enzymatic properties of an aminopeptidase from barley.

8. Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.

9. Purification and properties of a proteolytic enzyme from French beans.

10. Estimation of the molecular weights of proteins by Sephadex gel-filtration.

1. Physiological control of exo- and endoproteolytic activities in germinating wheat and their relationship to storage protein hydrolysis.

2. The role of cysteine proteinase and carboxypeptidase in the breakdown of storage proteins in buckwheat seeds.

3. Patterns of proteolytic enzyme activities in different tissues of germinating corn (Zea mays L.).

4. Specificity of the carboxypeptidase inhibitor from potatoes.

5. Intracellular Localization of Peptide Hydrolases in Wheat (Triticum aestivum L.) Leaves.

6. Hemoglobin-digesting Acid Proteinases in Soybean Leaves: CHARACTERISTICS AND CHANGES DURING LEAF MATURATION AND SENESCENCE.

7. Proteolytic and Trypsin Inhibitor Activity in Germinating Jojoba Seeds (Simmondsia chinensis).

8. Autodigestion in crude extracts of soybean leaves and isolated chloroplasts as a measure of proteolytic activity.

9. Acid Carboxypeptidases in Grains and Leaves of Wheat, Triticum aestivum L.

10. Proteases and Peptidases of Castor Bean Endosperm: Enzyme Characterization and Changes during Germination.