An amphoteric conjugate of [h]gibberellin a(1) from barley aleurone layers.

The major metabolite produced during incubation of [(3)H]gibberellin A(1) ([(3)H]GA(1)) with barley aleurone layers is an amphoteric, water-soluble compound tentatively called [(3)H]ampho GA(1). Formation of [(3)H]ampho GA(1) in barley aleurones begins after a period of 2.5 hours. As judged by degradation studies as well as Sephadex column chromatography, GA(1) appears to be linked to a peptide; positions C-3 and C-7 were ruled out as conjugation sites.