| Literature DB >> 16658720 |
Abstract
An NAD-linked dehydrogenase from Chlorella pyrenoidosa Chick catalyzing the conversion of l-proline to Delta(1)-pyrroline-5-carboxylic acid was partially purified. Delta(1)-Pyrroline-5-carboxylic acid was identified as the product by co-chromatography of it and its o-aminobenzaldehyde derivative with authentic compounds. The enzyme is NAD and l-proline specific and is not an oxidase; NADP is not inhibitory. The Michaelis constant for NAD is 0.08 mm and for proline is 0.73 mm.Entities:
Year: 1974 PMID: 16658720 PMCID: PMC543244 DOI: 10.1104/pp.53.3.440
Source DB: PubMed Journal: Plant Physiol ISSN: 0032-0889 Impact factor: 8.340