Literature DB >> 16657242

Nature of the Conversion of Ficus carica Variety Kadota Ficin Component D to Component C. Some Physicochemical Properties of Components C and D.

D E Kramer1, J R Whitaker.   

Abstract

Component C can be formed from component D under the experimental conditions used during purification of Ficus carica variety Kadota latex. By use of the inhibitor, sodium p-chloromercuribenzoate, the 2 components have been purified to chromatographic homogeneity. The 2 components have identical molecular weights and amino acid composition. The only difference found between the 2 components is the presence of 3 to 6 more amide groups in component D than in component C. There also appears to be a conformational difference between the 2 since component C is not as acidic, with respect to component D, as would be expected from the comparative amide contents. Conformational differences between the 2 are also indicated by the chromatographic behavior of the 2 in the presence and absence of sodium-p-chloromercuribenzoate.

Entities:  

Year:  1969        PMID: 16657242      PMCID: PMC396307          DOI: 10.1104/pp.44.11.1566

Source DB:  PubMed          Journal:  Plant Physiol        ISSN: 0032-0889            Impact factor:   8.340


  7 in total

1.  FICUS ENZYMES. I. SEPARATION OF THE PROTEOLYTIC ENZYMES OF FICUS CARICA AND FICUS GLABRATA LATICES.

Authors:  V C SGARBIERI; S M GUPTE; D E KRAMER; J R WHITAKER
Journal:  J Biol Chem       Date:  1964-07       Impact factor: 5.157

2.  ON THE MOLECULAR WEIGHTS OF THE PROTEOLYTIC ENZYMES OF STEM BROMELAIN.

Authors:  G FEINSTEIN; J R WHITAKER
Journal:  Biochemistry       Date:  1964-08       Impact factor: 3.162

3.  Ficincatalysed reactions: the affinity of ficin for some arginine derivatives.

Authors:  S A BERNHARD; H GUTFREUND
Journal:  Biochem J       Date:  1956-05       Impact factor: 3.857

4.  Multiple molecular forms of ficin-evidence against autolysis as explanation.

Authors:  D E Kramer; J R Whitaker
Journal:  Plant Physiol       Date:  1969-11       Impact factor: 8.340

5.  Proteolytic activity in the genus ficus.

Authors:  D C Williams; V C Sgarbieri; J R Whitaker
Journal:  Plant Physiol       Date:  1968-07       Impact factor: 8.340

6.  Studies on ficin. I. Its isolation and characterization.

Authors:  P T Englund; T P King; L C Craig; A Walti
Journal:  Biochemistry       Date:  1968-01       Impact factor: 3.162

7.  Inhibition of ficin by the chloromethyl ketone derivatives of n-tosyl-l-lysine and n-tosyl-l-phenylalanine.

Authors:  M J Stein; I E Liener
Journal:  Biochem Biophys Res Commun       Date:  1967-02-08       Impact factor: 3.575

  7 in total
  4 in total

1.  Multiple Molecular Forms of Ficus glabrata Ficin. Their Separation and Relative Physical, Chemical, and Enzymatic Properties.

Authors:  D C Williams; J R Whitaker
Journal:  Plant Physiol       Date:  1969-11       Impact factor: 8.340

2.  Partial Purification and Characterization of a Phytochrome-degrading Neutral Protease from Etiolated Oat Shoots.

Authors:  C S Pike; W R Briggs
Journal:  Plant Physiol       Date:  1972-04       Impact factor: 8.340

3.  Multiple molecular forms of ficin-evidence against autolysis as explanation.

Authors:  D E Kramer; J R Whitaker
Journal:  Plant Physiol       Date:  1969-11       Impact factor: 8.340

4.  Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex.

Authors:  Hamid Zare; Ali Akbar Moosavi-Movahedi; Maryam Salami; Morteza Mirzaei; Ali Akbar Saboury; Nader Sheibani
Journal:  Phytochemistry       Date:  2013-01-10       Impact factor: 4.072

  4 in total

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