Literature DB >> 16652994

A new method of measuring protein-methionine-s-oxide reductase activity.

D L Ferguson1, J J Burke.   

Abstract

A new assay for measuring protein-methionine-S-oxide reductase is reported. The assay measures the conversion of N-(9-fluorenylmethoxycarbonyl)-methionine sulfoxide to N-(9-fluorenylmethoxycarbonyl)-methionine using fluorescence detection after high-performance liquid chromatography separation. Enzyme activity is linear over a 60-min period and the assay is sensitive enough to detect the consumption of only 1% of available substrate. Protein-methionine-S-oxide reductase activity was demonstrated in pea (Pisum sativum L.) chloroplasts, and enzyme levels in leaves of etiolated and light-grown seedlings were compared.

Entities:  

Year:  1992        PMID： 16652994      PMCID： PMC1075582          DOI： 10.1104/pp.100.1.529

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  10 in total

1.  Evidence for membrane protein oxidation during in vivo aging of human erythrocytes.

Authors:  C Seppi; M A Castellana; G Minetti; G Piccinini; C Balduini; A Brovelli
Journal:  Mech Ageing Dev       Date:  1991-03       Impact factor: 5.432

2.  The oxidative inactivation of human alpha-1-proteinase inhibitor. Further evidence for methionine at the reactive center.

Authors:  D Johnson; J Travis
Journal:  J Biol Chem       Date:  1979-05-25       Impact factor: 5.157

3.  The synthesis and analytical use of a highly sensitive and convenient substrate of elastase.

Authors:  J Bieth; B Spiess; C G Wermuth
Journal:  Biochem Med       Date:  1974-12

4.  Reduction of N-acetyl methionine sulfoxide: a simple assay for peptide methionine sulfoxide reductase.

Authors:  N Brot; J Werth; D Koster; H Weissbach
Journal:  Anal Biochem       Date:  1982-05-15       Impact factor: 3.365

5.  Enzymatic reduction of oxidized alpha-1-proteinase inhibitor restores biological activity.

Authors:  W R Abrams; G Weinbaum; L Weissbach; H Weissbach; N Brot
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

6.  Reduction of N-acetyl methionine sulfoxide in plants.

Authors:  J Sánchez; B J Nikolau; P K Stumpf
Journal:  Plant Physiol       Date:  1983-11       Impact factor: 8.340

7.  Methionine sulfoxide formation: the cause of self-inactivation of reticulocyte lipoxygenase.

Authors:  S Rapoport; B Härtel; G Hausdorf
Journal:  Eur J Biochem       Date:  1984-03-15

8.  Measurement of protein using bicinchoninic acid.

Authors:  P K Smith; R I Krohn; G T Hermanson; A K Mallia; F H Gartner; M D Provenzano; E K Fujimoto; N M Goeke; B J Olson; D C Klenk
Journal:  Anal Biochem       Date:  1985-10       Impact factor: 3.365

9.  Enzymatic reduction of protein-bound methionine sulfoxide.

Authors:  N Brot; L Weissbach; J Werth; H Weissbach
Journal:  Proc Natl Acad Sci U S A       Date:  1981-04       Impact factor: 11.205

10.  Superoxide production in aprotic interior of chloroplast thylakoids.

Authors:  M Takahashi; K Asada
Journal:  Arch Biochem Biophys       Date:  1988-12       Impact factor: 4.013
  10 in total
  3 in total

1.  Investigations into the role of the plastidial peptide methionine sulfoxide reductase in response to oxidative stress in Arabidopsis.

Authors:  Hernán M Romero; Barbara S Berlett; Philip J Jensen; Eva J Pell; Ming Tien
Journal:  Plant Physiol       Date:  2004-10-29       Impact factor: 8.340

Review 2.  Physiological Roles of Plant Methionine Sulfoxide Reductases in Redox Homeostasis and Signaling.

Authors:  Pascal Rey; Lionel Tarrago
Journal:  Antioxidants (Basel)       Date:  2018-08-29

3.  Structural basis of protein oxidation resistance: a lysozyme study.

Authors:  Marion Girod; Quentin Enjalbert; Claire Brunet; Rodolphe Antoine; Jérôme Lemoine; Iva Lukac; Miroslav Radman; Anita Krisko; Philippe Dugourd
Journal:  PLoS One       Date:  2014-07-07       Impact factor: 3.240
  3 in total

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