OBJECTIVE: The high temperature requirement factor A (HTRA) family consists of serine proteases with domains homologous to those of bacterial HTRA. Four human HTRA members have been described: HTRA1-4. HTRA1 and HTRA3 share a high degree of domain homologies and may therefore share a functional similarity. HTRA1 mRNA and protein is reported to be down-regulated in SV40-transformed cells, a malignant melanoma cell line, ovarian tumors, and ovarian cancer cell lines, suggesting a progressive loss of HTRA1 and the protein in cancer. This raises the possibility that HTRA3 may likewise be involved in cancer. This study examined the expression of mRNA and protein levels of HTRA1 and HTRA3 in human endometrial cancer (EC). METHODS: Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was performed in normal endometrium (n = 4) and in three grades of EC (n = 5 for each EC grade). Immunohistochemistry was used to determine the protein expression and the cellular localization of HTRA1 and HTRA3 in normal endometrium tissue (n = 6) and in three grades of EC (n = 8-10 for each EC grade). RESULTS: RT-PCR analysis showed a significant reduction of HTRA1 and HTRA3 mRNA in endometrial cancer compared to normal endometrium. HTRA1 and HTRA3 protein showed a similar pattern of expression in EC tissue. Positive immunostaining, scored semiquantitatively, revealed a significant decrease of HTRA1 and 3 protein expression with increasing grades of EC. CONCLUSION: These data suggest that HTRA1 and HTRA3 mRNA and protein levels decrease with increasing grades of EC.
OBJECTIVE: The high temperature requirement factor A (HTRA) family consists of serine proteases with domains homologous to those of bacterial HTRA. Four humanHTRA members have been described: HTRA1-4. HTRA1 and HTRA3 share a high degree of domain homologies and may therefore share a functional similarity. HTRA1 mRNA and protein is reported to be down-regulated in SV40-transformed cells, a malignant melanoma cell line, ovarian tumors, and ovarian cancer cell lines, suggesting a progressive loss of HTRA1 and the protein in cancer. This raises the possibility that HTRA3 may likewise be involved in cancer. This study examined the expression of mRNA and protein levels of HTRA1 and HTRA3 in humanendometrial cancer (EC). METHODS: Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was performed in normal endometrium (n = 4) and in three grades of EC (n = 5 for each EC grade). Immunohistochemistry was used to determine the protein expression and the cellular localization of HTRA1 and HTRA3 in normal endometrium tissue (n = 6) and in three grades of EC (n = 8-10 for each EC grade). RESULTS: RT-PCR analysis showed a significant reduction of HTRA1 and HTRA3 mRNA in endometrial cancer compared to normal endometrium. HTRA1 and HTRA3 protein showed a similar pattern of expression in EC tissue. Positive immunostaining, scored semiquantitatively, revealed a significant decrease of HTRA1 and 3 protein expression with increasing grades of EC. CONCLUSION: These data suggest that HTRA1 and HTRA3 mRNA and protein levels decrease with increasing grades of EC.
Authors: Sally A Mullany; Mehdi Moslemi-Kebria; Ramandeep Rattan; Ashwani Khurana; Amy Clayton; Takayo Ota; Andrea Mariani; Karl C Podratz; Jeremy Chien; Viji Shridhar Journal: Clin Cancer Res Date: 2010-11-23 Impact factor: 12.531
Authors: Angela Chamberland; Eunice Wang; Aled R Jones; Lisa A Collins-Racie; Edward R LaVallie; Ying Huang; Lin Liu; Elisabeth A Morris; Carl R Flannery; Zhiyong Yang Journal: J Biol Chem Date: 2009-08-05 Impact factor: 5.157
Authors: Velia D'Angelo; Giulia Pecoraro; Paolo Indolfi; Adriana Iannotta; Vittoria Donofrio; Maria Elena Errico; Cristiana Indolfi; Maria Ramaglia; Angela Lombardi; Martina Di Martino; Vincenzo Gigantino; Alfonso Baldi; Michele Caraglia; Antonio De Luca; Fiorina Casale Journal: J Neurooncol Date: 2014-02-04 Impact factor: 4.130
Authors: Anna Lehner; Viktor Magdolen; Tibor Schuster; Matthias Kotzsch; Marion Kiechle; Alfons Meindl; Fred C G J Sweep; Paul N Span; Eva Gross Journal: PLoS One Date: 2013-04-08 Impact factor: 3.240