| Literature DB >> 16647707 |
Jeffrey S Batten1, Benedicte Desvoyes, Yoshimi Yamamura, Karen-Beth G Scholthof.
Abstract
Panicum mosaic virus (PMV) is a single-stranded positive-sense RNA virus in the family Tombusviridae. PMV genomic RNA (gRNA) and subgenomic RNA (sgRNA) are not capped or polyadenylated. We have determined that PMV uses a cap-independent mechanism of translation. A 116-nucleotide translational enhancer (TE) region on the 3'-untranslated region of both the gRNA and sgRNA has been identified. The TE is required for efficient translation of viral proteins in vitro. For mutants with a compromised TE, addition of cap analog, or transposition of the cis-active TE to another location, both restored translational competence of the 5'-proximal sgRNA genes in vitro.Entities:
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Year: 2006 PMID： 16647707 DOI： 10.1016/j.febslet.2006.04.006
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124