| Literature DB >> 16632251 |
Linquan Bai1, Lei Li, Hui Xu, Kazuyuki Minagawa, Yi Yu, Yirong Zhang, Xiufen Zhou, Heinz G Floss, Taifo Mahmud, Zixin Deng.
Abstract
A 45 kb DNA sequencing analysis from Streptomyces hygroscopicus 5008 involved in validamycin A (VAL-A) biosynthesis revealed 16 structural genes, 2 regulatory genes, 5 genes related transport, transposition/integration or tellurium resistance; another 4 genes had no obvious identity. The VAL-A biosynthetic pathway was proposed, with assignment of the required genetic functions confined to the sequenced region. A cluster of eight reassembled genes was found to support VAL-A synthesis in a heterologous host, S. lividans 1326. In vivo inactivation of the putative glycosyltransferase gene (valG) abolished the final attachment of glucose for VAL production and resulted in accumulation of the VAL-A precursor, validoxylamine, while the normal production of VAL-A could be restored by complementation with valG. The role of valG in the glycosylation of validoxylamine to VAL-A was demonstrated in vitro by enzymatic assay.Entities:
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Year: 2006 PMID： 16632251 PMCID： PMC1474575 DOI： 10.1016/j.chembiol.2006.02.002
Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521