Literature DB >> 16619113

Efficient genetic transformation system for the ochratoxigenic fungus Aspergillus carbonarius.

Luiz Rodrigo I Morioka1, Márcia C Furlaneto, Andréa C Bogas, Patrícia Pompermayer, Rubens T D Duarte, Maria Lúcia C Vieira, Maria Angélica Ehara Watanabe, Maria Helena P Fungaro.   

Abstract

Aspergillus carbonarius is a potent ochratoxin A producer that has been found in products such as grapes, coffee, spices, and cocoa. Ochratoxin A has nephrotoxic effect, and it has been classified as a possible carcinogenic substance for humans. Here we describe for the first time a transformation system for A. carbonarius, providing an important step towards its genetic manipulation. Conidia were transformed to acquire hygromycin B resistance using the AGL-1 strain of Agrobacterium tumefaciens. Genetic transformation was evaluated growing A. tumefaciens cells in induction medium containing or not acetosyringone prior to co-cultivation. The mean transforming efficiencies in IM+AS and IM-AS conditions were 62.2 and 44.5 transformants per 10(5) conidia, respectively. The hph gene was random integrated into the genome of A. carbonarius. Fungal sequences flanking the insertion site could be amplified by TAIL-PCR.

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Year:  2006        PMID： 16619113     DOI： 10.1007/s00284-005-0402-6

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  17 in total

1.  Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR.

Authors:  Y G Liu; N Mitsukawa; T Oosumi; R F Whittier
Journal:  Plant J       Date:  1995-09       Impact factor: 6.417

Review 2.  Agrobacterium-mediated transformation as a tool for functional genomics in fungi.

Authors:  Caroline B Michielse; Paul J J Hooykaas; Cees A M J J van den Hondel; Arthur F J Ram
Journal:  Curr Genet       Date:  2005-05-12       Impact factor: 3.886

3.  What is the source of ochratoxin A in wine?

Authors:  F J Cabañes; F Accensi; M R Bragulat; M L Abarca; G Castellá; S Minguez; A Pons
Journal:  Int J Food Microbiol       Date:  2002-12-15       Impact factor: 5.277

4.  Fungal flora and ochratoxin a production in grapes and musts from france.

Authors:  Lucile Sage; Serge Krivobok; Emilie Delbos; Françoise Seigle-Murandi; Edmond E Creppy
Journal:  J Agric Food Chem       Date:  2002-02-27       Impact factor: 5.279

5.  Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in the symbiotic ectomycorrhizal fungus Hebeloma cylindrosporum.

Authors:  Jean-Philippe Combier; Delphine Melayah; Colette Raffier; Gilles Gay; Roland Marmeisse
Journal:  FEMS Microbiol Lett       Date:  2003-03-14       Impact factor: 2.742

6.  Efficient transformation of Neurospora crassa by utilizing hybrid plasmid DNA.

Authors:  M E Case; M Schweizer; S R Kushner; N H Giles
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

7.  Occurrence of ochratoxin A-producing fungi in grapes grown in Italy.

Authors:  Paola Battilani; Amedeo Pietri; Terenzio Bertuzzi; Luca Languasco; Paola Giorni; Zofia Kozakiewicz
Journal:  J Food Prot       Date:  2003-04       Impact factor: 2.077

8.  Agrobacterium tumefaciens-mediated transformation of filamentous fungi.

Authors:  M J de Groot; P Bundock; P J Hooykaas; A G Beijersbergen
Journal:  Nat Biotechnol       Date:  1998-09       Impact factor: 54.908

9.  The source of ochratoxin A in Brazilian coffee and its formation in relation to processing methods.

Authors:  M H Taniwaki; J I Pitt; A A Teixeira; B T Iamanaka
Journal:  Int J Food Microbiol       Date:  2003-04-25       Impact factor: 5.277

10.  Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae.

Authors:  P Bundock; A den Dulk-Ras; A Beijersbergen; P J Hooykaas
Journal:  EMBO J       Date:  1995-07-03       Impact factor: 11.598
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  4 in total

1.  Genetic transformation of Diaporthe phaseolorum, an endophytic fungus found in mangrove forests, mediated by Agrobacterium tumefaciens.

Authors:  Fernanda L S Sebastianes; Paulo T Lacava; Léia C L Fávaro; Maria B C Rodrigues; Welington L Araújo; João L Azevedo; Aline A Pizzirani-Kleiner
Journal:  Curr Genet       Date:  2011-12-31       Impact factor: 3.886

2.  Introduction of large DNA inserts into the barley pathogenic fungus, Ustilago hordei, via recombined binary BAC vectors and Agrobacterium-mediated transformation.

Authors:  Shawkat Ali; Guus Bakkeren
Journal:  Curr Genet       Date:  2010-10-10       Impact factor: 3.886

3.  Agrobacterium tumefaciens-mediated genetic transformation of the phytopathogenic fungus Penicillium digitatum.

Authors:  Ji-Ye Wang; Hong-Ye Li
Journal:  J Zhejiang Univ Sci B       Date:  2008-10       Impact factor: 3.066

4.  Genetic transformation of Fusarium avenaceum by Agrobacterium tumefaciens mediated transformation and the development of a USER-Brick vector construction system.

Authors:  Lisette Quaade Sørensen; Erik Lysøe; Jesper Erup Larsen; Paiman Khorsand-Jamal; Kristian Fog Nielsen; Rasmus John Normand Frandsen
Journal:  BMC Mol Biol       Date:  2014-07-22       Impact factor: 2.946
  4 in total

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