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Literature DB >> 16617121

Methods of quantitative proteomics and their application to plant organelle characterization.

Abstract

Many cell biologists wish to know the subcellular localization of proteins of interest. Proteomics methods have the potential to describe the entire protein content of organelles. However, practical limitations in organelle isolation and analysis of low abundance proteins have meant that organelle proteomics has had, until recently, only limited success. Some examples of quantitative proteomic methods and their use in the study of plant organelle proteomes are discussed here. It is concluded that 2D-difference gel electrophoresis (2D-DIGE) as well as differential isotope tagging strategies coupled to non-gel-based LC-MS are proving useful in this area of research.

Mesh：

Substances：
Arabidopsis Proteins

Year: 2006 PMID： 16617121 DOI： 10.1093/jxb/erj141

Source DB: PubMed Journal: J Exp Bot ISSN： 0022-0957 Impact factor: 6.992

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Cited

12 in total

1. Proteomic identification of toxic volatile organic compound-responsive proteins in Arabidopsis thaliana.

Authors: Min-Ah Park; Jae-Hyun Seo; Jong-Sug Park; Mi Kwon
Journal: Plant Cell Rep Date: 2009-08-21 Impact factor: 4.570

2. A predicted physicochemically distinct sub-proteome associated with the intracellular organelle of the anammox bacterium Kuenenia stuttgartiensis.

Authors: Marnix H Medema; Miaomiao Zhou; Sacha A F T van Hijum; Jolein Gloerich; Hans J C T Wessels; Roland J Siezen; Marc Strous
Journal: BMC Genomics Date: 2010-05-12 Impact factor: 3.969

3. A proteomics study of brassinosteroid response in Arabidopsis.

Authors: Zhiping Deng; Xin Zhang; Wenqiang Tang; Juan A Oses-Prieto; Nagi Suzuki; Joshua M Gendron; Huanjing Chen; Shenheng Guan; Robert J Chalkley; T Kaye Peterman; Alma L Burlingame; Zhi-Yong Wang
Journal: Mol Cell Proteomics Date: 2007-09-11 Impact factor: 5.911

Review 4. Proteomic approaches to the characterization of protein thiol modification.

Authors: Edward T Chouchani; Andrew M James; Ian M Fearnley; Kathryn S Lilley; Michael P Murphy
Journal: Curr Opin Chem Biol Date: 2010-12-02 Impact factor: 8.822

5. Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation.

Authors: Edward T Chouchani; Thomas R Hurd; Sergiy M Nadtochiy; Paul S Brookes; Ian M Fearnley; Kathryn S Lilley; Robin A J Smith; Michael P Murphy
Journal: Biochem J Date: 2010-08-15 Impact factor: 3.857

6. Proteomic analysis of peach fruit mesocarp softening and chilling injury using difference gel electrophoresis (DIGE).

Authors: Ricardo Nilo; Carlos Saffie; Kathryn Lilley; Ricardo Baeza-Yates; Verónica Cambiazo; Reinaldo Campos-Vargas; Mauricio González; Lee A Meisel; Julio Retamales; Herman Silva; Ariel Orellana
Journal: BMC Genomics Date: 2010-01-18 Impact factor: 3.969

Methods of quantitative proteomics and their application to plant organelle characterization.

1. Proteomic identification of toxic volatile organic compound-responsive proteins in Arabidopsis thaliana.

2. A predicted physicochemically distinct sub-proteome associated with the intracellular organelle of the anammox bacterium Kuenenia stuttgartiensis.

3. A proteomics study of brassinosteroid response in Arabidopsis.

Review 4. Proteomic approaches to the characterization of protein thiol modification.

5. Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation.

6. Proteomic analysis of peach fruit mesocarp softening and chilling injury using difference gel electrophoresis (DIGE).

7. Current progress in tonoplast proteomics reveals insights into the function of the large central vacuole.

8. Integration of shot-gun proteomics and bioinformatics analysis to explore plant hormone responses.

9. Identification of trans-golgi network proteins in Arabidopsis thaliana root tissue.

10. Visualization of Multicolored in vivo Organelle Markers for Co-Localization Studies in Oryza sativa.