Literature DB >> 16617080

Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells.

Dylan M Owen1, Peter M P Lanigan, Christopher Dunsby, Ian Munro, David Grant, Mark A A Neil, Paul M W French, Anthony I Magee.   

Abstract

We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700 ps lifetime shift between liquid-disordered and liquid-ordered phases offers greater contrast than the 60 nm spectral shift previously reported. Detection of cholesterol-rich membrane microdomains is confirmed by observation of the temperature dependence of membrane order and by cholesterol depletion using methyl-beta-cyclodextrin.

Entities:  

Mesh：

Substances：

Year:  2006        PMID： 16617080      PMCID： PMC1459501          DOI： 10.1529/biophysj.106.084673

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  7 in total

1.  Integrin-dependent interaction of lipid rafts with the actin cytoskeleton in activated human platelets.

Authors:  Stéphane Bodin; Carine Soulet; Hélène Tronchère; Pierre Sié; Christian Gachet; Monique Plantavid; Bernard Payrastre
Journal:  J Cell Sci       Date:  2005-01-25       Impact factor: 5.285

2.  Cholesterol-enriched lipid domains can be visualized by di-4-ANEPPDHQ with linear and nonlinear optics.

Authors:  Lei Jin; Andrew C Millard; Joseph P Wuskell; Heather A Clark; Leslie M Loew
Journal:  Biophys J       Date:  2005-05-06       Impact factor: 4.033

Review 3.  Functional rafts in cell membranes.

Authors:  K Simons; E Ikonen
Journal:  Nature       Date:  1997-06-05       Impact factor: 49.962

4.  Novel naphthylstyryl-pyridium potentiometric dyes offer advantages for neural network analysis.

Authors:  A L Obaid; L M Loew; J P Wuskell; B M Salzberg
Journal:  J Neurosci Methods       Date:  2004-04-30       Impact factor: 2.390

5.  Sphingomyelin/phosphatidylcholine/cholesterol phase diagram: boundaries and composition of lipid rafts.

Authors:  Rodrigo F M de Almeida; Aleksandre Fedorov; Manuel Prieto
Journal:  Biophys J       Date:  2003-10       Impact factor: 4.033

6.  Visualizing lipid structure and raft domains in living cells with two-photon microscopy.

Authors:  Katharina Gaus; Enrico Gratton; Eleanor P W Kable; Allan S Jones; Ingrid Gelissen; Leonard Kritharides; Wendy Jessup
Journal:  Proc Natl Acad Sci U S A       Date:  2003-12-12       Impact factor: 11.205

Review 7.  The state of lipid rafts: from model membranes to cells.

Authors:  Michael Edidin
Journal:  Annu Rev Biophys Biomol Struct       Date:  2003-01-16
  7 in total
  40 in total

1.  Imaging membrane lipid order in whole, living vertebrate organisms.

Authors:  Dylan M Owen; Astrid Magenau; Arindam Majumdar; Katharina Gaus
Journal:  Biophys J       Date:  2010-07-07       Impact factor: 4.033

Review 2.  Fluorescence lifetime measurements and biological imaging.

Authors:  Mikhail Y Berezin; Samuel Achilefu
Journal:  Chem Rev       Date:  2010-05-12       Impact factor: 60.622

Review 3.  Dynamic organization of lymphocyte plasma membrane: lessons from advanced imaging methods.

Authors:  Dylan M Owen; Katharina Gaus; Anthony I Magee; Marek Cebecauer
Journal:  Immunology       Date:  2010-07-15       Impact factor: 7.397

4.  Using Time-Resolved Fluorescence Anisotropy of di-4-ANEPPDHQ and F2N12S to Analyze Lipid Packing Dynamics in Model Systems.

Authors:  Harmen B B Steele; Matthew J Sydor; Donald S Anderson; Andrij Holian; J B Alexander Ross
Journal:  J Fluoresc       Date:  2019-04-01       Impact factor: 2.217

5.  Visualization of membrane rafts using a perylene monoimide derivative and fluorescence lifetime imaging.

Authors:  Anca Margineanu; Jun-ichi Hotta; Renaud A L Vallée; Mark Van der Auweraer; Marcel Ameloot; Alina Stefan; David Beljonne; Yves Engelborghs; Andreas Herrmann; Klaus Müllen; Frans C De Schryver; Johan Hofkens
Journal:  Biophys J       Date:  2007-06-15       Impact factor: 4.033

6.  Fluorescence lifetime imaging of membrane lipid order with a ratiometric fluorescent probe.

Authors:  Vasyl Kilin; Oleksandr Glushonkov; Lucas Herdly; Andrey Klymchenko; Ludovic Richert; Yves Mely
Journal:  Biophys J       Date:  2015-05-19       Impact factor: 4.033

7.  Soluble klotho binds monosialoganglioside to regulate membrane microdomains and growth factor signaling.

Authors:  George Dalton; Sung-Wan An; Saif I Al-Juboori; Nicole Nischan; Joonho Yoon; Evgenia Dobrinskikh; Donald W Hilgemann; Jian Xie; Kate Luby-Phelps; Jennifer J Kohler; Lutz Birnbaumer; Chou-Long Huang
Journal:  Proc Natl Acad Sci U S A       Date:  2017-01-09       Impact factor: 11.205

8.  The phenyltetraene lysophospholipid analog PTE-ET-18-OMe as a fluorescent anisotropy probe of liquid ordered membrane domains (lipid rafts) and ceramide-rich membrane domains.

Authors:  Omar Bakht; Javier Delgado; Francisco Amat-Guerri; A Ulises Acuña; Erwin London
Journal:  Biochim Biophys Acta       Date:  2007-05-13

9.  Amino(oligo)thiophene-based environmentally sensitive biomembrane chromophores.

Authors:  Ping Yan; Aifang Xie; Meide Wei; Leslie M Loew
Journal:  J Org Chem       Date:  2008-07-30       Impact factor: 4.354

10.  Modification of plasma membrane organization in tobacco cells elicited by cryptogein.

Authors:  Patricia Gerbeau-Pissot; Christophe Der; Dominique Thomas; Iulia-Andra Anca; Kevin Grosjean; Yann Roche; Jean-Marie Perrier-Cornet; Sébastien Mongrand; Françoise Simon-Plas
Journal:  Plant Physiol       Date:  2013-11-14       Impact factor: 8.340
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.