Literature DB >> 16614906

Cloning, expression, and characterization of a DNA ligase from a hyperthermophilic archaeon Thermococcus sp.

Yun Jae Kim1, Hyun Sook Lee, Seung Seob Bae, Jung Ho Jeon, Sung Hyun Yang, Jae Kyu Lim, Sung Gyun Kang, Suk-Tae Kwon, Jung-Hyun Lee.   

Abstract

Genomic analysis of a hyperthermophilic archaeon, Thermococcus sp. NA1, revealed an ORF of 1689 bases encoding 562 amino acids that showed a high similarity to DNA ligases from other hyperthermophilic archaea. The ligase, which was designated TNA1_lig (Thermococcus sp. NA1 ligase), was cloned and expressed in Escherichia coli. The recombinant TNA1_lig was purified by metal affinity chromatography. The optimum ligase activity of the recombinant TNA1_lig occurred at 80 degrees C and pH 7.5. The enzyme was activated by MgCl2 and ZnCl2 but was inhibited by MnCl2 and NiCl2. Additionally, the enzyme was activated by either ATP or NAD+.

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Year:  2006        PMID： 16614906     DOI： 10.1007/s10529-005-6070-6

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  8 in total

1.  Expression, purification, crystallization and preliminary crystallographic analysis of a thermostable DNA ligase from the archaeon Thermococcus sibiricus.

Authors:  T E Petrova; E Y Bezsudnova; B D Dorokhov; E S Slutskaya; K M Polyakov; P V Dorovatovskiy; N V Ravin; K G Skryabin; M V Kovalchuk; V O Popov
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-01-25

2.  Purification, crystallization and preliminary crystallographic analysis of a multiple cofactor-dependent DNA ligase from Sulfophobococcus zilligii.

Authors:  Supangat Supangat; Young Jun An; Younguk Sun; Suk-Tae Kwon; Sun-Shin Cha
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-11-16

3.  A single amino acid substitution in the DNA-binding domain of Aeropyrum pernix DNA ligase impairs its interaction with proliferating cell nuclear antigen.

Authors:  Shinichi Kiyonari; Toru Kamigochi; Yoshizumi Ishino
Journal:  Extremophiles       Date:  2007-05-09       Impact factor: 2.395

4.  ATP-dependent DNA ligase from Thermococcus sp. 1519 displays a new arrangement of the OB-fold domain.

Authors:  T Petrova; E Y Bezsudnova; K M Boyko; A V Mardanov; K M Polyakov; V V Volkov; M Kozin; N V Ravin; I G Shabalin; K G Skryabin; T N Stekhanova; M V Kovalchuk; V O Popov
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-11-14

5.  Broad nucleotide cofactor specificity of DNA ligase from the hyperthermophilic crenarchaeon Hyperthermus butylicus and its evolutionary significance.

Authors:  Jun-Hwan Kim; Kang-Keun Lee; Younguk Sun; Gang-Jin Seo; Sung Suk Cho; Suk Hyung Kwon; Suk-Tae Kwon
Journal:  Extremophiles       Date:  2013-04-02       Impact factor: 3.035

6.  Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation.

Authors:  Shih-Hsun Chen; Xiaochun Yu
Journal:  Nucleic Acids Res       Date:  2019-02-20       Impact factor: 16.971

Review 7.  Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology.

Authors:  Cecilia R Chambers; Wayne M Patrick
Journal:  Archaea       Date:  2015-10-01       Impact factor: 3.273

Review 8.  Biotechnological applications of archaeal enzymes from extreme environments.

Authors:  Ma Ángeles Cabrera; Jenny M Blamey
Journal:  Biol Res       Date:  2018-10-05       Impact factor: 5.612
  8 in total

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