A gene similar to the human hyaluronan-mediated motility receptor (RHAMM) gene is upregulated during Porcine Circovirus type 2 infection.

Little is known on the cellular events triggered by the Porcine Circovirus type 2 (PCV2) in Porcine Multisystemic Wasting Syndrome (PMWS). The differential display reverse-transcription PCR (DDRT-PCR) was used to identify cellular target molecules in lymph node tissue that were regulated in PMWS. Comparative profile analysis of a pool of lymph node tissues from PMWS and healthy animals showed that some transcripts were up-regulated in PMWS. Bacterial recombinant clones containing up-regulated transcripts were analyzed by reverse dot blot. Clones showing enhanced hybridization when probed with cDNAs from PMWS animals were sequenced and compared to existing databases. Two of the differentially regulated transcripts displayed homology with human genes such as an RNA splicing factor and hyaluronan-mediated motility receptor (RHAMM). Clones encoding theses genes were subsequently used as probes to analyze their expression pattern in PK15 cells persistently infected with PCV2. Northern blot analyzes indicated that these transcripts were up-regulated in these cells as observed in infected lymph node tissue from PMWS cases. A role for the up-regulation of the RHAMM gene is proposed.