| Literature DB >> 10227473 |
G M Allan1, F Mc Neilly, B M Meehan, S Kennedy, D P Mackie, J A Ellis, E G Clark, E Espuna, N Saubi, P Riera, A Bøtner, C E Charreyre.
Abstract
A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1).Entities:
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Year: 1999 PMID： 10227473 DOI： 10.1016/s0378-1135(99)00004-8
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293