Literature DB >> 1659686

High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110.

R D Everett1, A Orr, M Elliott.   

Abstract

Herpes simplex virus type 1 (HSV-1) encodes five immediate early (IE) polypeptides. This paper reports the construction of a baculovirus vector which expresses large amounts of Vmw110, the product of IE gene 1. The expressed protein has been purified to near homogeneity and has a mobility on SDS polyacrylamide gels identical to that of Vmw110 produced during HSV-1 infection. Characterisation of its properties indicated that it forms dimers and perhaps higher order oligomers in solution and that the purified protein binds to both single stranded and double stranded calf thymus DNA cellulose columns. However, filter binding experiments were unable to detect any stable association of Vmw110 with DNA in solution.

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Year:  1991        PMID： 1659686      PMCID： PMC329111          DOI： 10.1093/nar/19.22.6155

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  45 in total

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5.  A novel cysteine-rich sequence motif.

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  37 in total

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10.  The activation region of the Tat protein of human immunodeficiency virus type-1 functions in yeast.

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