Literature DB >> 16593998

Identification of a unique isoform of 1-aminocyclopropane-1-carboxylic acid synthase by monoclonal antibody.

A M Mehta1, R L Jordan, J D Anderson, A K Mattoo.   

Abstract

1-Aminocyclopropane-1-carboxylic acid (ACC) synthase (EC 4.4.1.14) is a key enzyme regulating ethylene biosynthesis in higher plants. A monoclonal antibody (mAb T20C) that immunoprecipitates the ACC synthase activity from tomato pericarp tissue extracts revealed that mAb T20C immunodecorates an approximately 67-kDa polypeptide. On isoelectric focusing gels, ACC synthase activity in cell-free preparations was resolved into three distinct activity peaks with pI values 5.3, 7, and 9. mAb T20C specifically recognized the pI 7 form of the enzyme on electrophoretic transfer (Western) blots. When analyzed by sodium dodecyl sulfate gel electrophoresis under reducing conditions, the eluted pI 7 form was confirmed to migrate as a polypeptide of 67 kDa. The 67-kDa pI 7 isoform is a previously undescribed form of ACC synthase.

Entities:  

Year:  1988        PMID： 16593998      PMCID： PMC282596          DOI： 10.1073/pnas.85.23.8810

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  12 in total

1.  Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris.

Authors:  K E Broglie; J J Gaynor; R M Broglie
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Radiolabeling of a wound-inducible pyridoxal phosphate-utilizing enzyme: evidence for its identification as ACC synthase.

Authors:  L S Privalle; J S Graham
Journal:  Arch Biochem Biophys       Date:  1987-03       Impact factor: 4.013

6.  Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.

Authors:  C R Merril; D Goldman; S A Sedman; M H Ebert
Journal:  Science       Date:  1981-03-27       Impact factor: 47.728

7.  A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid.

Authors:  M C Lizada; S F Yang
Journal:  Anal Biochem       Date:  1979-11-15       Impact factor: 3.365

8.  Monoclonal antibodies against HLA products and their use in immunoaffinity purification.

Authors:  P Parham
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

9.  A quantitative dot-immunobinding assay for proteins using nitrocellulose membrane filters.

Authors:  R Jahn; W Schiebler; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

10.  Use of monoclonal antibodies in the purification and characterization of 1-aminocyclopropane-1-carboxylate synthase, an enzyme in ethylene biosynthesis.

Authors:  A B Bleecker; W H Kenyon; S C Somerville; H Kende
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205
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  10 in total

1.  Enzymes of ethylene biosynthesis.

Authors:  H Kende
Journal:  Plant Physiol       Date:  1989-09       Impact factor: 8.340

2.  Wound-regulated accumulation of specific transcripts in tomato fruit: interactions with fruit development, ethylene and light.

Authors:  B L Parsons; A K Mattoo
Journal:  Plant Mol Biol       Date:  1991-09       Impact factor: 4.076

3.  Cloning and sequence of two different cDNAs encoding 1-aminocyclopropane-1-carboxylate synthase in tomato.

Authors:  D Van der Straeten; L Van Wiemeersch; H M Goodman; M Van Montagu
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

4.  A 22-bp deletion in OsPLS3 gene encoding a DUF266-containing protein is implicated in rice leaf senescence.

Authors:  Kunyu Li; Yaodong Chen; Yanmin Luo; Fudeng Huang; Chaoyue Zhao; Fangmin Cheng; Xun Xiang; Gang Pan
Journal:  Plant Mol Biol       Date:  2018-08-16       Impact factor: 4.076

5.  Cloning the mRNA encoding 1-aminocyclopropane-1-carboxylate synthase, the key enzyme for ethylene biosynthesis in plants.

Authors:  T Sato; A Theologis
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

6.  Purification and characterization of 1-aminocyclopropane-1-carboxylate synthase from apple fruits.

Authors:  W K Yip; J G Dong; S F Yang
Journal:  Plant Physiol       Date:  1991-01       Impact factor: 8.340

7.  Translational modification of an 18 kilodalton polypeptide by spermidine in rice cell suspension cultures.

Authors:  A M Mehta; R A Saftner; G W Schaeffer; A K Mattoo
Journal:  Plant Physiol       Date:  1991-04       Impact factor: 8.340

8.  Characterization of three members of the ACC synthase gene family in Solanum tuberosum L.

Authors:  L J Destéfano-Beltrán; W van Caeneghem; J Gielen; L Richard; M van Montagu; D van der Straeten
Journal:  Mol Gen Genet       Date:  1995-02-20

9.  Accumulation of wound-inducible ACC synthase transcript in tomato fruit is inhibited by salicylic acid and polyamines.

Authors:  N Li; B L Parsons; D R Liu; A K Mattoo
Journal:  Plant Mol Biol       Date:  1992-02       Impact factor: 4.076

10.  Effects of Flooding and Endogenous Hormone on the Formation of Knee Roots in Taxodium ascendens.

Authors:  Zhuangzhuang Qian; Lin Wu; Luozhong Tang
Journal:  Front Plant Sci       Date:  2022-02-03       Impact factor: 5.753
  10 in total

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