Literature DB >> 1658389

Assembly of double-shelled rotaviruslike particles by simultaneous expression of recombinant VP6 and VP7 proteins.

M Sabara1, M Parker, P Aha, C Cosco, E Gibbons, S Parsons, L A Babiuk.   

Abstract

Simultaneous coinfection of Spodoptera frugiperda cells with baculovirus recombinants containing the genes for VP6 and VP7 of bovine rotavirus strain C486 resulted in the production of spherical particles resembling smooth, double-shelled rotavirus. These particles were predominantly located in the cell culture supernatant instead of being cell associated. Pretreatment of infected-cell culture supernatants with CaCl2, prior to particle purification, was found to enhance the smooth appearance of the particles. The authenticity of the proteins making up the particles was demonstrated by their reactivity with antiserum specific for double-shelled C486 rotavirus.

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Year:  1991        PMID： 1658389      PMCID： PMC250814     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  14 in total

1.  Preliminary characterization of an epitope involved in neutralization and cell attachment that is located on the major bovine rotavirus glycoprotein.

Authors:  M Sabara; J E Gilchrist; G R Hudson; L A Babiuk
Journal:  J Virol       Date:  1985-01       Impact factor: 5.103

2.  In vitro assembly of bovine rotavirus nucleocapsid protein.

Authors:  K F Ready; M Sabara
Journal:  Virology       Date:  1987-03       Impact factor: 3.616

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Cloning and sequence of UK bovine rotavirus gene segment 7: marked sequence homology with simian rotavirus gene segment 8.

Authors:  M L Dyall-Smith; T C Elleman; P A Hoyne; I H Holmes; A A Azad
Journal:  Nucleic Acids Res       Date:  1983-05-25       Impact factor: 16.971

5.  Assembly of double-shelled, viruslike particles of bluetongue virus by the simultaneous expression of four structural proteins.

Authors:  T J French; J J Marshall; P Roy
Journal:  J Virol       Date:  1990-12       Impact factor: 5.103

6.  In vitro assembly of the outer capsid of bovine rotavirus is calcium-dependent.

Authors:  K F Ready; M I Sabara; L A Babiuk
Journal:  Virology       Date:  1988-11       Impact factor: 3.616

7.  Synthesis of immunogenic, but non-infectious, poliovirus particles in insect cells by a baculovirus expression vector.

Authors:  T Urakawa; M Ferguson; P D Minor; J Cooper; M Sullivan; J W Almond; D H Bishop
Journal:  J Gen Virol       Date:  1989-06       Impact factor: 3.891

8.  Expression of rotavirus VP2 produces empty corelike particles.

Authors:  M Labbé; A Charpilienne; S E Crawford; M K Estes; J Cohen
Journal:  J Virol       Date:  1991-06       Impact factor: 5.103

9.  Heterotypic passive protection induced by synthetic peptides corresponding to VP7 and VP4 of bovine rotavirus.

Authors:  M K Ijaz; S K Attah-Poku; M J Redmond; M D Parker; M I Sabara; P Frenchick; L A Babiuk
Journal:  J Virol       Date:  1991-06       Impact factor: 5.103

10.  Nucleotide sequence of bovine rotavirus gene 1 and expression of the gene product in baculovirus.

Authors:  J Cohen; A Charpilienne; S Chilmonczyk; M K Estes
Journal:  Virology       Date:  1989-07       Impact factor: 3.616
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  15 in total

1.  Interaction of rotavirus polymerase VP1 with nonstructural protein NSP5 is stronger than that with NSP2.

Authors:  F Arnoldi; M Campagna; C Eichwald; U Desselberger; O R Burrone
Journal:  J Virol       Date:  2006-12-20       Impact factor: 5.103

2.  Characterization and replicase activity of double-layered and single-layered rotavirus-like particles expressed from baculovirus recombinants.

Authors:  C Q Zeng; M J Wentz; J Cohen; M K Estes; R F Ramig
Journal:  J Virol       Date:  1996-05       Impact factor: 5.103

3.  In vitro reconstitution of rotavirus transcriptional activity using viral cores and recombinant baculovirus expressed VP6.

Authors:  E Kohli; P Pothier; G Tosser; J Cohen; A M Sandino; E Spencer
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

4.  High level production of potyvirus-like particles in insect cells infected with recombinant baculovirus.

Authors:  S J Edwards; M B Hayden; R C Hamilton; J A Haynes; I T Nisbet; M N Jagadish
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

5.  Strong buffering capacity of insect cells. Implications for the baculovirus expression system.

Authors:  M Medina; A López-Rivas; D Zuidema; G J Belsham; E Domingo; J M Vlak
Journal:  Cytotechnology       Date:  1995-02       Impact factor: 2.058

6.  Expression of rotavirus capsid protein VP6 in transgenic potato and its oral immunogenicity in mice.

Authors:  Jie Yu; William Langridge
Journal:  Transgenic Res       Date:  2003-04       Impact factor: 2.788

7.  Expression of the rotavirus SA11 protein VP7 in the simple eukaryote Dictyostelium discoideum.

Authors:  K R Emslie; J M Miller; M B Slade; P R Dormitzer; H B Greenberg; K L Williams
Journal:  J Virol       Date:  1995-03       Impact factor: 5.103

8.  Expression of human papillomavirus type 11 L1 protein in insect cells: in vivo and in vitro assembly of viruslike particles.

Authors:  R C Rose; W Bonnez; R C Reichman; R L Garcea
Journal:  J Virol       Date:  1993-04       Impact factor: 5.103

Review 9.  Self-assembling protein nanoparticles in the design of vaccines.

Authors:  Jacinto López-Sagaseta; Enrico Malito; Rino Rappuoli; Matthew J Bottomley
Journal:  Comput Struct Biotechnol J       Date:  2015-11-26       Impact factor: 7.271

10.  Recombinant outer capsid glycoprotein (VP7) of rotavirus expressed in insect cells induces neutralizing antibodies in rabbits.

Authors:  M Khodabandehloo; M Shamsi Shahrabadi; H Keyvani; B Bambai; Za Sadigh
Journal:  Iran J Public Health       Date:  2012-05-31       Impact factor: 1.429
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