BACKGROUND AND AIMS: Augmentation of collateral vessel growth (arteriogenesis) is of particular clinical interest for the treatment of vascular occlusive disease. Monocytes play a key role for arteriogenesis. They localize to areas of collateral development and create a highly arteriogenic environment. "Homing" of ex vivo genetically engineered monocytes could therapeutically be exploited for augmentation of arteriogenesis. However, isolation and ex vivo transduction of monocytes is problematic. METHODS: In this study, we established a valid method of monocyte isolation from peripheral blood and evaluated different in vitro transduction methods. RESULTS: Our results revealed that liposomes and electroporation were unsuccessful for monocyte transduction. However, high-efficiency gene transfer (almost 95%) was achieved by adenoviral infection. Subsequent homing of virally transduced monocytes to sites of arteriogenesis could be demonstrated. CONCLUSION: Our study may offer a new method for the augmentation of arteriogenesis, all of which makes the ultimate goal of applying this strategy to humans for therapy of vascular disease eminently attractive.
BACKGROUND AND AIMS: Augmentation of collateral vessel growth (arteriogenesis) is of particular clinical interest for the treatment of vascular occlusive disease. Monocytes play a key role for arteriogenesis. They localize to areas of collateral development and create a highly arteriogenic environment. "Homing" of ex vivo genetically engineered monocytes could therapeutically be exploited for augmentation of arteriogenesis. However, isolation and ex vivo transduction of monocytes is problematic. METHODS: In this study, we established a valid method of monocyte isolation from peripheral blood and evaluated different in vitro transduction methods. RESULTS: Our results revealed that liposomes and electroporation were unsuccessful for monocyte transduction. However, high-efficiency gene transfer (almost 95%) was achieved by adenoviral infection. Subsequent homing of virally transduced monocytes to sites of arteriogenesis could be demonstrated. CONCLUSION: Our study may offer a new method for the augmentation of arteriogenesis, all of which makes the ultimate goal of applying this strategy to humans for therapy of vascular disease eminently attractive.
Authors: Carmen Urbich; Christopher Heeschen; Alexandra Aicher; Elisabeth Dernbach; Andreas M Zeiher; Stefanie Dimmeler Journal: Circulation Date: 2003-10-27 Impact factor: 29.690
Authors: D L Fischman; M B Leon; D S Baim; R A Schatz; M P Savage; I Penn; K Detre; L Veltri; D Ricci; M Nobuyoshi Journal: N Engl J Med Date: 1994-08-25 Impact factor: 91.245
Authors: Jamal S Lewis; Natalia V Dolgova; Thomas J Chancellor; Abhinav P Acharya; Jerome V Karpiak; Tanmay P Lele; Benjamin G Keselowsky Journal: Biomaterials Date: 2013-09-03 Impact factor: 12.479
Authors: Martin Wagner; Adrian Mahlmann; Elisabeth Deindl; Werner Zuschratter; Monika Riek-Burchardt; Sawa Kostin; Blerim Luani; Claudia Baer; Akram Youssef; Joerg Herold Journal: Am J Transl Res Date: 2019-07-15 Impact factor: 4.060
Authors: Martin Wagner; Helen Koester; Christian Deffge; Soenke Weinert; Johannes Lauf; Alexander Francke; Jerry Lee; R C Braun-Dullaeus; Joerg Herold Journal: J Vis Exp Date: 2014-12-27 Impact factor: 1.355
Authors: Alexander Francke; Soenke Weinert; Ruth H Strasser; Ruediger C Braun-Dullaeus; Joerg Herold Journal: Am J Transl Res Date: 2013-03-28 Impact factor: 4.060