COMPARISON OF SPHEROPLAST INDUCTION IN ALCALIGENES FAECALIS BY THREE DIFFERENT AGENTS.

Lark, Cynthia (Saint Louis University, St. Louis, Mo.) and Robert Schichtel. Comparison of spheroplast induction in Alcaligenes faecalis by three different agents. J. Bacteriol. 84:1241-1244. 1962.-Alcaligenes faecalis strain LB was exposed to different concentrations of cycloserine, d-methionine, or penicillin. The time course of spheroplast induction by these agents was measured as a function of their concentration. The results were consistent with models in which cycloserine reversibly inhibited an intracellular enzyme, and d-methionine was built into a defective cell-wall unit. Penicillin simulated cycloserine. This was taken as further evidence that penicillin inhibits an enzyme associated with cell-wall synthesis.