| Literature DB >> 16554154 |
Zhengying Pan1, Douglas A Jeffery, Kareem Chehade, Jerlyn Beltman, James M Clark, Paul Grothaus, Matthew Bogyo, Amos Baruch.
Abstract
A series of diphenylphosphonate-based probes were developed for the trypsin-like serine proteases. These probes selectively target serine proteases rather than general serine hydrolases that are targets for fluorophosphonate-based probes. This increased selectivity allows detection of low abundance serine proteases in complex proteomes using simple SDS-PAGE methods. We present here the application of multiple probes in enzyme activity profiling of intact mast cells, a type of inflammatory cell implicated in allergy and autoimmune diseases.Entities:
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Year: 2006 PMID： 16554154 DOI： 10.1016/j.bmcl.2006.03.012
Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823