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Literature DB >> 16550460

Cloning, sequencing and characterization of a novel phosphatase gene, phoI, from soil bacterium Enterobacter sp. 4.

Seung Ha Kang¹, Kwang Keun Cho, Jin Duck Bok, Sung Chan Kim, Jaie Soon Cho, Peter Chang-Whan Lee, Sang Kee Kang, Hong Gu Lee, Jung Hee Woo, Hyun Jeong Lee, Sang Cheol Lee, Yun Jaie Choi.

Abstract

A gene, phoI, coding for a phosphatase from Enterobacter sp. 4 was cloned in Escherichia coli and sequenced. Analysis of the sequence revealed one open reading frame (ORF) that encodes a 269-amino acid protein with a calculated molecular mass of 29 kDa. PhoI belongs to family B acid phosphatase and exhibits 49.4% identity and 62.4% homology to the hel gene from Heamophilus influenzae, which encoded an outer membrane protein (P4). The optimum pH and temperature for phosphatase activity were pH 5.5 and 40 degrees C, respectively. Its specific activity on rho-nitrophenyl phosphatate was 70 U/mg at pH 5.5 and 40 degrees C. Enzyme activity was inhibited by Al3+, EDTA, and DTT, but fivefold activated by Cu2+ ion (350 U/mg). PhoI showed a strong synergistic effect when used with a purified E. coli phytase, AppA, to estimate combination effects.

Entities: Chemical

Mesh：

Substances：

Year: 2006 PMID： 16550460 DOI： 10.1007/s00284-005-4467-z

Source DB: PubMed Journal: Curr Microbiol ISSN： 0343-8651 Impact factor: 2.343

23 in total

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Journal: Med Microbiol Immunol Date: 1997-06 Impact factor: 3.402

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Journal: Biochim Biophys Acta Date: 1988-06-09

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Authors: A H Ullah
Journal: Prep Biochem Date: 1988

6. Acid phosphatase-1 from nematode resistant tomato : isolation and characterization of its gene.

Authors: V M Williamson; G Colwell
Journal: Plant Physiol Date: 1991-09 Impact factor: 8.340

7. The e (P4) outer membrane protein of Haemophilus influenzae: biologic activity of anti-e serum and cloning and sequencing of the structural gene.

Authors: B A Green; J E Farley; T Quinn-Dey; R A Deich; G W Zlotnick
Journal: Infect Immun Date: 1991-09 Impact factor: 3.441

Cloning, sequencing and characterization of a novel phosphatase gene, phoI, from soil bacterium Enterobacter sp. 4.

1. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT.

2. The lppC gene of Streptococcus equisimilis encodes a lipoprotein that is homologous to the e (P4) outer membrane protein from Haemophilus influenzae.

3. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts.

Review 4. Transcending the impenetrable: how proteins come to terms with membranes.

5. Production, rapid purification and catalytic characterization of extracellular phytase from Aspergillus ficuum.

6. Acid phosphatase-1 from nematode resistant tomato : isolation and characterization of its gene.

7. The e (P4) outer membrane protein of Haemophilus influenzae: biologic activity of anti-e serum and cloning and sequencing of the structural gene.

8. Cloning, characterization and overexpression of the phytase-encoding gene (phyA) of Aspergillus niger.

9. DNA sequencing with chain-terminating inhibitors.

10. Identification and cloning of a second phytase gene (phyB) from Aspergillus niger (ficuum).

1. Kinetic characterization of a novel acid ectophosphatase from Enterobacter asburiae.

2. A novel phytase appA from Citrobacter amalonaticus CGMCC 1696: gene cloning and overexpression in Pichia pastoris.