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Literature DB >> 16533850

Explanation of the stability of thermophilic proteins based on unique micromorphology.

Simone Melchionna¹, Raffaele Sinibaldi, Giuseppe Briganti.

Abstract

Two mesophilic/thermophilic variants of the G-domain of the elongation factor Tu were studied via molecular dynamics simulations. By analyzing the simulation data via the Voronoi space tessellation, we have found that the two proteins have the same macromolecular packing, while the water-exposed surface area is larger for the thermophile. A larger coordination with water is probably due to a peculiar corrugation of the exposed surface of this species. From an enthalpic point of view, the thermophile shows a larger number of intramolecular hydrogen bonds, stronger electrostatic interactions, and a flatter free-energy landscape. Overall, the data suggest that the specific hydration state enhances macromolecular fluctuations but, at the same time, increases thermal stability.

Entities: Chemical Gene

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Year: 2006 PMID： 16533850 PMCID： PMC1459513 DOI： 10.1529/biophysj.105.078972

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

43 in total

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Authors: P J Haney; J H Badger; G L Buldak; C I Reich; C R Woese; G J Olsen
Journal: Proc Natl Acad Sci U S A Date: 1999-03-30 Impact factor: 11.205

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Authors: A A Pantazaki; A A Pritsa; D A Kyriakidis
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Authors: David Chandler
Journal: Nature Date: 2002-05-30 Impact factor: 49.962

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Journal: J Mol Biol Date: 1998-11-27 Impact factor: 5.469

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Authors: T Lazaridis; I Lee; M Karplus
Journal: Protein Sci Date: 1997-12 Impact factor: 6.725

8. Crystal structure of the EF-Tu.EF-Ts complex from Thermus thermophilus.

Authors: Y Wang; Y Jiang; M Meyering-Voss; M Sprinzl; P B Sigler
Journal: Nat Struct Biol Date: 1997-08

9. Hydrophobic collapse in multidomain protein folding.

Authors: Ruhong Zhou; Xuhui Huang; Claudio J Margulis; Bruce J Berne
Journal: Science Date: 2004-09-10 Impact factor: 47.728

10. Water induced effects on the thermal response of a protein.

Authors: Simone Melchionna; Giuseppe Briganti; Paola Londei; Piero Cammarano
Journal: Phys Rev Lett Date: 2004-04-13 Impact factor: 9.161

12 in total

1. Crowding induces differences in the diffusion of thermophilic and mesophilic proteins: a new look at neutron scattering results.

Authors: Enrique Marcos; Pau Mestres; Ramon Crehuet
Journal: Biophys J Date: 2011-12-07 Impact factor: 4.033

2. Thermo- and mesostabilizing protein interactions identified by temperature-dependent statistical potentials.

Authors: Benjamin Folch; Yves Dehouck; Marianne Rooman
Journal: Biophys J Date: 2010-02-17 Impact factor: 4.033

3. X-ray radiolytic labeling reveals the molecular basis of orange carotenoid protein photoprotection and its interactions with fluorescence recovery protein.

Authors: Sayan Gupta; Markus Sutter; Soumya G Remesh; Maria Agustina Dominguez-Martin; Han Bao; Xinyu A Feng; Leanne-Jade G Chan; Christopher J Petzold; Cheryl A Kerfeld; Corie Y Ralston
Journal: J Biol Chem Date: 2019-04-12 Impact factor: 5.157

Explanation of the stability of thermophilic proteins based on unique micromorphology.

1. Thermal adaptation analyzed by comparison of protein sequences from mesophilic and extremely thermophilic Methanococcus species.

Review 2. Biotechnologically relevant enzymes from Thermus thermophilus.

3. Hydrophobicity: two faces of water.

Review 4. Heat capacity in proteins.

5. The stability of salt bridges at high temperatures: implications for hyperthermophilic proteins.

6. Heat capacity of proteins. II. Partial molar heat capacity of the unfolded polypeptide chain of proteins: protein unfolding effects.

7. Dynamics and unfolding pathways of a hyperthermophilic and a mesophilic rubredoxin.

8. Crystal structure of the EF-Tu.EF-Ts complex from Thermus thermophilus.

9. Hydrophobic collapse in multidomain protein folding.

10. Water induced effects on the thermal response of a protein.

1. Crowding induces differences in the diffusion of thermophilic and mesophilic proteins: a new look at neutron scattering results.

2. Thermo- and mesostabilizing protein interactions identified by temperature-dependent statistical potentials.

3. X-ray radiolytic labeling reveals the molecular basis of orange carotenoid protein photoprotection and its interactions with fluorescence recovery protein.

Review 4. Thermostability engineering of industrial enzymes through structure modification.

Review 5. Thermophilic proteins: insight and perspective from in silico experiments.

6. Role of Internal Water on Protein Thermal Stability: The Case of Homologous G Domains.

7. Integrated Structural Studies for Elucidating Carotenoid-Protein Interactions.

8. Stability curve prediction of homologous proteins using temperature-dependent statistical potentials.

Review 9. Slow unfolding of monomeric proteins from hyperthermophiles with reversible unfolding.

10. Stay Wet, Stay Stable? How Internal Water Helps the Stability of Thermophilic Proteins.