| Literature DB >> 16530175 |
Anne Bertrand1, Sandrine Morel, François Lefoulon, Yves Rolland, Pierre Monsan, Magali Remaud-Simeon.
Abstract
The enzymatic glucosylation of luteolin was attempted using two glucansucrases: the dextransucrase from Leuconostoc mesenteroides NRRL B-512F and the alternansucrase from L. mesenteroides NRRL B-23192. Reactions were carried out in aqueous-organic solvents to improve luteolin solubility. A molar conversion of 44% was achieved after 24h of reaction catalysed by dextransucrase from L. mesenteroides NRRL B-512F in a mixture of acetate buffer (70%)/bis(2-methoxyethyl) ether (30%). Two products were characterised by nuclear magnetic resonance (NMR) spectroscopy: luteolin-3'-O-alpha-d-glucopyranoside and luteolin-4'-O-alpha-d-glucopyranoside. In the presence of alternansucrase from L. mesenteroides NRRL B-23192, three additional products were obtained with a luteolin conversion of 8%. Both enzymes were also able to glucosylate quercetin and myricetin with conversion of 4% and 49%, respectively.Entities:
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Year: 2006 PMID： 16530175 DOI： 10.1016/j.carres.2006.02.008
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104