| Literature DB >> 16524725 |
Rajendra Kumar Saxena1, Kakoli Dutt, Lata Agarwal, Preeti Nayyar.
Abstract
A highly thermostable alkaline amylase producing Bacillus sp. PN5 was isolated from soil, which yielded 65.23 U mL(-1) of amylase in medium containing (%) 0.6 starch, 0.5 peptone and 0.3 yeast extract at 60 degrees C, pH 7.0 after 60 h of incubation. Maximum amylase activity was at pH 10.0 and 90 degrees C. The enzyme retained 80% activity after 1 h at pH 10.0. It exhibited 65% activity at 105 degrees C and had 100% stability in the temperature range between 80 and 100 degrees C for 1 h. In addition, there was 86.36% stability after 1-h incubation with sodium dodecylsulphate. These properties indicated possible use of this amylase in starch saccharification and detergent formulation.Entities:
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Year: 2006 PMID： 16524725 DOI： 10.1016/j.biortech.2006.01.016
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642