PURPOSE: Lateral fluid percussion brain injury (FPI) increases cyclooxygenase-2 (COX-2) expression in the cortex and hippocampus. The objective was to investigate whether the selective COX-2 inhibitor rofecoxib (10 mg/kg twice daily) reduces neuronal cell death after FPI in rats, since rofecoxib has been shown to be neuroprotective in other models of CNS injury. METHODS: Rofecoxib (n = 23) or vehicle (n = 20) were administered after FPI and for up to 3 days. Cell death was evaluated by Fluoro-Jade B staining and by the TdT-mediated dUTP nick end labelling (TUNEL) assay. RESULTS: COX-2 immunoreactivity increased in the ipsilateral cortex and hippocampus (CA1) and bilaterally in the dentate gyri. Fluoro-Jade B- and TUNEL-positive cells were detected 12-72 h after FPI in the ipsilateral cortex and bilaterally in the dentate gyri. Fluoro-Jade B staining did not indicate a significant neuroprotective effect of rofecoxib (12-72 h) and neither did TUNEL staining. Quantificaton of the TUNEL staining in the ipsilateral cortex was approximately 50% lower in the rofecoxib group at 12 and 24 h, but this did not reach statistical significance (p = 0.06), and appeared unchanged at 72 h. CONCLUSIONS: Rofecoxib lacked significant protective effect on early neuronal cell death in the FPI model of traumatic brain injury.
PURPOSE: Lateral fluid percussion brain injury (FPI) increases cyclooxygenase-2 (COX-2) expression in the cortex and hippocampus. The objective was to investigate whether the selective COX-2 inhibitor rofecoxib (10 mg/kg twice daily) reduces neuronal cell death after FPI in rats, since rofecoxib has been shown to be neuroprotective in other models of CNS injury. METHODS:Rofecoxib (n = 23) or vehicle (n = 20) were administered after FPI and for up to 3 days. Cell death was evaluated by Fluoro-Jade B staining and by the TdT-mediated dUTP nick end labelling (TUNEL) assay. RESULTS:COX-2 immunoreactivity increased in the ipsilateral cortex and hippocampus (CA1) and bilaterally in the dentate gyri. Fluoro-Jade B- and TUNEL-positive cells were detected 12-72 h after FPI in the ipsilateral cortex and bilaterally in the dentate gyri. Fluoro-Jade B staining did not indicate a significant neuroprotective effect of rofecoxib (12-72 h) and neither did TUNEL staining. Quantificaton of the TUNEL staining in the ipsilateral cortex was approximately 50% lower in the rofecoxib group at 12 and 24 h, but this did not reach statistical significance (p = 0.06), and appeared unchanged at 72 h. CONCLUSIONS:Rofecoxib lacked significant protective effect on early neuronal cell death in the FPI model of traumatic brain injury.
Authors: Wenjin Li; Shasha Wu; Muzamil Ahmad; Jianfei Jiang; Hao Liu; Tetsuya Nagayama; Marie E Rose; Vladimir A Tyurin; Yulia Y Tyurina; Grigory G Borisenko; Natalia Belikova; Jun Chen; Valerian E Kagan; Steven H Graham Journal: J Neurochem Date: 2010-03-17 Impact factor: 5.372
Authors: Muzamil Ahmad; Marie E Rose; Vincent Vagni; Raymond P Griffith; C Edward Dixon; Patrick M Kochanek; Robert W Hickey; Steven H Graham Journal: J Neurosci Res Date: 2008-12 Impact factor: 4.164
Authors: Kayvan Dehlaghi Jadid; Johan Davidsson; Erik Lidin; Anders Hånell; Maria Angéria; Tiit Mathiesen; Mårten Risling; Mattias Günther Journal: Front Neurol Date: 2019-07-30 Impact factor: 4.003