BACKGROUND: A defined geographical area (Oxford, United Kingdom) was investigated for the role of group B Streptococcus (GBS) as a human pathogen. METHODS: GBS carriage in pregnant women and invasive disease in neonates and adults >60 years of age was studied over a 3-year period. Multilocus sequence typing and capsular serotyping were used to study 369 isolates of GBS from carriage in pregnant women (n=190) and invasive disease in neonates (n=109) and adults >60 years of age (n=70). RESULTS: A total of 20.3% of pregnant women carried GBS. Invasive GBS disease occurred at a rate of 0.9 cases per 1000 live births and 11 cases per 100,000 population >60 years of age per annum. Four sequence types (STs) (ST-17, ST-19, ST-23, and ST-1) that were identified with use of multilocus sequence typing accounted for >50% of carried and invasive strains. A single sequence type (ST-17), previously shown to be phylogenetically of bovine origin, was significantly associated with increased invasiveness in neonates (P=.00002), and this was independent of capsular serotype III. In contrast, among adults >60 years of age, no STs exhibited increased invasiveness, compared with STs carried in pregnant women. CONCLUSIONS: Enhanced invasiveness associated with ST-17 is specific to neonates and is independent of capsular serotype.
BACKGROUND: A defined geographical area (Oxford, United Kingdom) was investigated for the role of group B Streptococcus (GBS) as a human pathogen. METHODS:GBS carriage in pregnant women and invasive disease in neonates and adults >60 years of age was studied over a 3-year period. Multilocus sequence typing and capsular serotyping were used to study 369 isolates of GBS from carriage in pregnant women (n=190) and invasive disease in neonates (n=109) and adults >60 years of age (n=70). RESULTS: A total of 20.3% of pregnant women carried GBS. Invasive GBS disease occurred at a rate of 0.9 cases per 1000 live births and 11 cases per 100,000 population >60 years of age per annum. Four sequence types (STs) (ST-17, ST-19, ST-23, and ST-1) that were identified with use of multilocus sequence typing accounted for >50% of carried and invasive strains. A single sequence type (ST-17), previously shown to be phylogenetically of bovine origin, was significantly associated with increased invasiveness in neonates (P=.00002), and this was independent of capsular serotype III. In contrast, among adults >60 years of age, no STs exhibited increased invasiveness, compared with STs carried in pregnant women. CONCLUSIONS: Enhanced invasiveness associated with ST-17 is specific to neonates and is independent of capsular serotype.
Authors: Shannon Weiman; Satoshi Uchiyama; Feng-Ying C Lin; Donald Chaffin; Ajit Varki; Victor Nizet; Amanda L Lewis Journal: Biochem J Date: 2010-05-13 Impact factor: 3.857
Authors: Margaret Ip; Edmund S C Cheuk; Michelle H Y Tsui; Fanrong Kong; T N Leung; Gwendolyn L Gilbert Journal: J Clin Microbiol Date: 2006-09-27 Impact factor: 5.948
Authors: John F Bohnsack; April Whiting; Marcelo Gottschalk; Diane Marie Dunn; Robert Weiss; Parvin H Azimi; Joseph B Philips; Leonard E Weisman; George G Rhoads; Feng-Ying C Lin Journal: J Clin Microbiol Date: 2008-02-20 Impact factor: 5.948
Authors: Shannon D Manning; A Cody Springman; Amber D Million; Nicole R Milton; Sara E McNamara; Patricia A Somsel; Paul Bartlett; H Dele Davies Journal: PLoS One Date: 2010-01-20 Impact factor: 3.240