Literature DB >> 16511261

Crystallization, preliminary X-ray diffraction and structure solution of MosA, a dihydrodipicolinate synthase from Sinorhizobium meliloti L5-30.

Yvonne A Leduc1, Christopher P Phenix, Jennifer Puttick, Kurt Nienaber, David R J Palmer, Louis T J Delbaere.   

Abstract

The structure of MosA, a dihydrodipicolinate synthase and reported methyltransferase from Sinorhizobium meliloti, has been solved using molecular replacement with Escherichia coli dihydrodipicolinate synthase as the model. A crystal grown in the presence of pyruvate diffracted X-rays to 2.3 A resolution using synchrotron radiation and belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 69.14, b = 138.87, c = 124.13 A.

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Year:  2005        PMID: 16511261      PMCID: PMC2150934          DOI: 10.1107/S1744309105040893

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN: 1744-3091


  21 in total

1.  The CCP4 suite: programs for protein crystallography.

Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

2.  Novel buffer systems for macromolecular crystallization.

Authors:  Janet Newman
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-02-25

3.  The preparation of (S)-aspartate semi-aldehyde appropriate for use in biochemical studies.

Authors:  Sarah J Roberts; Jonathan C Morris; Renwick C J Dobson; Juliet A Gerrard
Journal:  Bioorg Med Chem Lett       Date:  2003-01-20       Impact factor: 2.823

4.  The crystal structures of native and (S)-lysine-bound dihydrodipicolinate synthase from Escherichia coli with improved resolution show new features of biological significance.

Authors:  Renwick C J Dobson; Michael D W Griffin; Geoffrey B Jameson; Juliet A Gerrard
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-07-20

5.  Amino-acid cycling drives nitrogen fixation in the legume-Rhizobium symbiosis.

Authors:  E M Lodwig; A H F Hosie; A Bourdès; K Findlay; D Allaway; R Karunakaran; J A Downie; P S Poole
Journal:  Nature       Date:  2003-04-17       Impact factor: 49.962

6.  Early production of rhizopine in nodules induced by Sinorhizobium meliloti strain L5-30.

Authors:  K Heinrich; M H Ryder; P J Murphy
Journal:  Can J Microbiol       Date:  2001-02       Impact factor: 2.419

7.  Dihydrodipicolinate synthase (DHDPS) from Escherichia coli displays partial mixed inhibition with respect to its first substrate, pyruvate.

Authors:  Renwick C J Dobson; Michael D W Griffin; Sarah J Roberts; Juliet A Gerrard
Journal:  Biochimie       Date:  2004 Apr-May       Impact factor: 4.079

8.  MosA, a protein implicated in rhizopine biosynthesis in Sinorhizobium meliloti L5-30, is a dihydrodipicolinate synthase.

Authors:  Pui Hang Tam; Christopher P Phenix; David R J Palmer
Journal:  J Mol Biol       Date:  2004-01-09       Impact factor: 5.469

9.  The crystal structure of three site-directed mutants of Escherichia coli dihydrodipicolinate synthase: further evidence for a catalytic triad.

Authors:  Renwick C J Dobson; Karin Valegård; Juliet A Gerrard
Journal:  J Mol Biol       Date:  2004-04-23       Impact factor: 5.469

10.  Dihydrodipicolinate synthase is not inhibited by its substrate, (S)-aspartate beta-semialdehyde.

Authors:  Renwick C J Dobson; Juliet A Gerrard; F Grant Pearce
Journal:  Biochem J       Date:  2004-02-01       Impact factor: 3.857

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