Literature DB >> 16511234

Improved expression, purification and crystallization of a putative N-acetyl-gamma-glutamyl-phosphate reductase from rice (Oryza sativa).

Jun Miura-Ohnuma1, Tsuyoshi Nonaka, Shizue Katoh, Katsuyoshi Murata, Akiko Kita, Kunio Miki, Etsuko Katoh.   

Abstract

N-Acetyl-gamma-glutamyl-phosphate reductase (AGPR) catalyzes the third step in an eight-step arginine-biosynthetic pathway that starts with glutamate. This enzyme converts N-acetyl-gamma-glutamyl phosphate to N-acetylglutamate-gamma-semialdehyde by an NADPH-dependent reductive dephosphorylation. AGPR from Oryza sativa (OsAGPR) was expressed in Escherichia coli at 291 K as a soluble fusion protein with an upstream thioredoxin-hexahistidine [Trx-(His)6] extension. OsAGPR(Ala50-Pro366) was purified and crystals were obtained using the sitting-drop vapour-diffusion method at 293 K and diffract X-rays to at least 1.8 A resolution. They belong to the hexagonal space group P6(1), with unit-cell parameters a = 86.11, c = 316.3 A.

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Year:  2005        PMID： 16511234      PMCID： PMC1978146          DOI： 10.1107/S1744309105035384

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  13 in total

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Review 5.  International Rice Genome Sequencing Project: the effort to completely sequence the rice genome.

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Journal:  Curr Opin Plant Biol       Date:  2000-04       Impact factor: 7.834

6.  Improving expression and solubility of rice proteins produced as fusion proteins in Escherichia coli.

Authors:  Yuki Tsunoda; Nobuya Sakai; Koji Kikuchi; Shizue Katoh; Kayo Akagi; Jun Miura-Ohnuma; Yumiko Tashiro; Katsuyoshi Murata; Naoto Shibuya; Etsuko Katoh
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8.  Expression, purification and preliminary X-ray characterization of N-acetyl-gamma-glutamyl-phosphate reductase from Thermus thermophilus HB8.

Authors:  Masaru Goto; Yoshihiro Agari; Rie Omi; Ikuko Miyahara; Ken Hirotsu
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2003-01-23

9.  Structure of holo-glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus at 1.8 A resolution.

Authors:  T Skarzyński; P C Moody; A J Wonacott
Journal:  J Mol Biol       Date:  1987-01-05       Impact factor: 5.469

10.  Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline.

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Journal:  Proc Natl Acad Sci U S A       Date:  2002-08-22       Impact factor: 11.205
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  1 in total

1.  Expression, purification, crystallization and preliminary X-ray analysis of two arginine-biosynthetic enzymes from Mycobacterium tuberculosis.

Authors:  Fatemeh Moradian; Craig Garen; Leonid Cherney; Maia Cherney; Michael N G James
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-09-30
  1 in total

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