Literature DB >> 16511206

Crystallization and preliminary crystallographic analysis of the human calcineurin homologous protein CHP2 bound to the cytoplasmic region of the Na+/H+ exchanger NHE1.

Youssef Ben Ammar1, Soichi Takeda, Mitsuaki Sugawara, Masashi Miyano, Hidezo Mori, Shigeo Wakabayashi.   

Abstract

Calcineurin homologous protein (CHP) is a Ca2+-binding protein that directly interacts with and regulates the activity of all plasma-membrane Na+/H+-exchanger (NHE) family members. In contrast to the ubiquitous isoform CHP1, CHP2 is highly expressed in cancer cells. To understand the regulatory mechanism of NHE1 by CHP2, the complex CHP2-NHE1 (amino acids 503-545) has been crystallized by the sitting-drop vapour-diffusion method using PEG 3350 as precipitant. The crystals diffract to 2.7 A and belong to a tetragonal space group, with unit-cell parameters a = b = 49.96, c = 103.20 A.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 16511206      PMCID： PMC1991313          DOI： 10.1107/S1744309105030836

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  17 in total

1.  The EF-hand Ca(2+)-binding protein p22 associates with microtubules in an N-myristoylation-dependent manner.

Authors:  S Timm; B Titus; K Bernd; M Barroso
Journal:  Mol Biol Cell       Date:  1999-10       Impact factor: 4.138

Review 2.  The expanding family of eucaryotic Na(+)/H(+) exchangers.

Authors:  L Counillon; J Pouysségur
Journal:  J Biol Chem       Date:  2000-01-07       Impact factor: 5.157

Review 3.  The changing face of the Na+/H+ exchanger, NHE1: structure, regulation, and cellular actions.

Authors:  L K Putney; S P Denker; D L Barber
Journal:  Annu Rev Pharmacol Toxicol       Date:  2002       Impact factor: 13.820

4.  Calcineurin homologous protein as an essential cofactor for Na+/H+ exchangers.

Authors:  T Pang; X Su; S Wakabayashi; M Shigekawa
Journal:  J Biol Chem       Date:  2001-02-28       Impact factor: 5.157

5.  Inhibition of calcineurin phosphatase activity by a calcineurin B homologous protein.

Authors:  X Lin; R A Sikkink; F Rusnak; D L Barber
Journal:  J Biol Chem       Date:  1999-12-17       Impact factor: 5.157

6.  A serine/threonine kinase which causes apoptosis-like cell death interacts with a calcineurin B-like protein capable of binding Na(+)/H(+) exchanger.

Authors:  M Matsumoto; Y Miyake; M Nagita; H Inoue; D Shitakubo; K Takemoto; C Ohtsuka; H Murakami; N Nakamura; H Kanazawa
Journal:  J Biochem       Date:  2001-08       Impact factor: 3.387

Review 7.  Diversity of the mammalian sodium/proton exchanger SLC9 gene family.

Authors:  John Orlowski; Sergio Grinstein
Journal:  Pflugers Arch       Date:  2003-07-04       Impact factor: 3.657

8.  Expression of calcineurin B homologous protein 2 protects serum deprivation-induced cell death by serum-independent activation of Na+/H+ exchanger.

Authors:  Tianxiang Pang; Shigeo Wakabayashi; Munekazu Shigekawa
Journal:  J Biol Chem       Date:  2002-09-10       Impact factor: 5.157

9.  KIF1Bbeta2, capable of interacting with CHP, is localized to synaptic vesicles.

Authors:  Norihiro Nakamura; Yoshihide Miyake; Masafumi Matsushita; Shingo Tanaka; Hiroki Inoue; Hiroshi Kanazawa
Journal:  J Biochem       Date:  2002-09       Impact factor: 3.387

10.  Role of calcineurin B homologous protein in pH regulation by the Na+/H+ exchanger 1: tightly bound Ca2+ ions as important structural elements.

Authors:  Tianxiang Pang; Takashi Hisamitsu; Hidezo Mori; Munekazu Shigekawa; Shigeo Wakabayashi
Journal:  Biochemistry       Date:  2004-03-30       Impact factor: 3.162
View more
  5 in total

1.  Crystal structure of CHP2 complexed with NHE1-cytosolic region and an implication for pH regulation.

Authors:  Youssef Ben Ammar; Soichi Takeda; Takashi Hisamitsu; Hidezo Mori; Shigeo Wakabayashi
Journal:  EMBO J       Date:  2006-05-18       Impact factor: 11.598

Review 2.  Trafficking Ion Transporters to the Apical Membrane of Polarized Intestinal Enterocytes.

Authors:  Amy Christine Engevik; James R Goldenring
Journal:  Cold Spring Harb Perspect Biol       Date:  2018-01-02       Impact factor: 10.005

Review 3.  NHE3 regulatory complexes.

Authors:  Mark Donowitz; Sachin Mohan; Cindy Xinjun Zhu; Tian-E Chen; Rong Lin; Boyoung Cha; Nicholas C Zachos; Rakhilya Murtazina; Rafiquel Sarker; Xuhang Li
Journal:  J Exp Biol       Date:  2009-06       Impact factor: 3.312

Review 4.  SLC9/NHE gene family, a plasma membrane and organellar family of Na⁺/H⁺ exchangers.

Authors:  Mark Donowitz; C Ming Tse; Daniel Fuster
Journal:  Mol Aspects Med       Date:  2013 Apr-Jun

5.  Involvement of CHP2 in the Development of Non-Small Cell Lung Cancer and Patients' Poor Prognosis.

Authors:  Liqin Xu; Yanmei Qin; Baier Sun; Haiying Wang; Jun Gu; Zhiyuan Tang; Weishuai Zhang; Jian Feng
Journal:  Appl Immunohistochem Mol Morphol       Date:  2020-10
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.