Literature DB >> 16511129

Crystallization and preliminary X-ray diffraction analysis of mouse 3(17)alpha-hydroxysteroid dehydrogenase.

Ossama El-Kabbani1, Syuhei Ishikura, Armin Wagner, Clemens Schulze-Briese, Akira Hara.   

Abstract

The 3(17)alpha-hydroxysteroid dehydrogenase from mouse is involved in the metabolism of oestrogens, androgens, neurosteroids and xenobiotic compounds. The enzyme was crystallized by the hanging-drop vapour-diffusion method in space group P222(1), with unit-cell parameters a = 84.91, b = 84.90, c = 95.83 A. The Matthews coefficient (VM) and the solvent content were 2.21 A3 Da(-1) and 44.6%, respectively, assuming the presence of two molecules in the asymmetric unit. Diffraction data were collected to a resolution of 1.8 A at the Swiss Light Source beamline X06SA using a MAR CCD area detector and gave a data set with an overall Rmerge of 6.8% and a completeness of 91.1%.

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Year:  2005        PMID： 16511129      PMCID： PMC1952465          DOI： 10.1107/S1744309105018427

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  16 in total

1.  Expression, crystallization and preliminary X-ray analysis of human and rabbit 20alpha-hydroxysteroid dehydrogenases in complex with NADP(H) and various steroid substrates.

Authors:  Jean François Couture; Line Cantin; Pierre Legrand; Van Luu-The; Fernand Labrie; Rock Breton
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2001-12-21

2.  Human 3alpha-hydroxysteroid dehydrogenase isoforms (AKR1C1-AKR1C4) of the aldo-keto reductase superfamily: functional plasticity and tissue distribution reveals roles in the inactivation and formation of male and female sex hormones.

Authors:  T M Penning; M E Burczynski; J M Jez; C F Hung; H K Lin; H Ma; M Moore; N Palackal; K Ratnam
Journal:  Biochem J       Date:  2000-10-01       Impact factor: 3.857

3.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

4.  Characterization of two isoforms of mouse 3(17)alpha-hydroxysteroid dehydrogenases of the aldo-keto reductase family.

Authors:  Syuhei Ishikura; Noriyuki Usami; Satoko Nakajima; Akiko Kameyama; Hiroaki Shiraishi; Vincenzo Carbone; Ossama El-Kabbani; Akira Hara
Journal:  Biol Pharm Bull       Date:  2004-12       Impact factor: 2.233

5.  Comparison of the multiple forms of the soluble 3(17) alpha-hydroxysteroid dehydrogenases of female rabbit kidney and liver.

Authors:  P C Lau; D S Layne; D G Williamson
Journal:  J Biol Chem       Date:  1982-08-25       Impact factor: 5.157

6.  Human cytosolic 3alpha-hydroxysteroid dehydrogenases of the aldo-keto reductase superfamily display significant 3beta-hydroxysteroid dehydrogenase activity: implications for steroid hormone metabolism and action.

Authors:  Stephan Steckelbroeck; Yi Jin; Sridhar Gopishetty; Busola Oyesanmi; Trevor M Penning
Journal:  J Biol Chem       Date:  2003-12-12       Impact factor: 5.157

7.  Human 20alpha-hydroxysteroid dehydrogenase: crystallographic and site-directed mutagenesis studies lead to the identification of an alternative binding site for C21-steroids.

Authors:  Jean-François Couture; Pierre Legrand; Line Cantin; Van Luu-The; Fernand Labrie; Rock Breton
Journal:  J Mol Biol       Date:  2003-08-15       Impact factor: 5.469

8.  Crystal structures of prostaglandin D(2) 11-ketoreductase (AKR1C3) in complex with the nonsteroidal anti-inflammatory drugs flufenamic acid and indomethacin.

Authors:  Andrew L Lovering; Jon P Ride; Christopher M Bunce; Julian C Desmond; Stephen M Cummings; Scott A White
Journal:  Cancer Res       Date:  2004-03-01       Impact factor: 12.701

9.  Loop relaxation, a mechanism that explains the reduced specificity of rabbit 20alpha-hydroxysteroid dehydrogenase, a member of the aldo-keto reductase superfamily.

Authors:  Jean-François Couture; Pierre Legrand; Line Cantin; Fernand Labrie; Van Luu-The; Rock Breton
Journal:  J Mol Biol       Date:  2004-05-21       Impact factor: 5.469

10.  A cluster of eight hydroxysteroid dehydrogenase genes belonging to the aldo-keto reductase supergene family on mouse chromosome 13.

Authors:  Laurent Vergnes; Jack Phan; Andrew Stolz; Karen Reue
Journal:  J Lipid Res       Date:  2002-12-01       Impact factor: 5.922
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  1 in total

1.  Structure of 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21) holoenzyme from an orthorhombic crystal form: an insight into the bifunctionality of the enzyme.

Authors:  Urmi Dhagat; Vincenzo Carbone; Roland P-T Chung; Clemens Schulze-Briese; Satoshi Endo; Akira Hara; Ossama El-Kabbani
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-09-19
  1 in total

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