Literature DB >> 16511040

Crystallization and X-ray diffraction analysis of dihydropyrimidinase from Saccharomyces kluyveri.

Doreen Dobritzsch1, Birgit Andersen, Jure Piskur.   

Abstract

Dihydropyrimidinase (EC 3.5.2.2) catalyzes the second step in the reductive pathway of pyrimidine degradation, the hydrolysis of 5,6-dihydrouracil and 5,6-dihydrothymine to the corresponding N-carbamylated beta-amino acids. Crystals of the recombinant enzyme from the yeast Saccharomyces kluyveri diffracting to 2.6 A at a synchrotron-radiation source have been obtained by the hanging-drop vapour-diffusion method. They belong to space group P2(1) (unit-cell parameters a = 91.0, b = 73.0, c = 161.4 A, beta = 91.4 degrees), with one homotetramer per asymmetric unit.

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Year:  2005        PMID： 16511040      PMCID： PMC1952421          DOI： 10.1107/S174430910500610X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  24 in total

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Review 2.  Microbial hydantoinases--industrial enzymes from the origin of life?

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4.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

Review 5.  Inborn errors of pyrimidine degradation: clinical, biochemical and molecular aspects.

Authors:  A H van Gennip; N G Abeling; P Vreken; A B van Kuilenburg
Journal:  J Inherit Metab Dis       Date:  1997-06       Impact factor: 4.982

Review 6.  Degradation of pyrimidines and pyrimidine analogs--pathways and mutual influences.

Authors:  C Wasternack
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8.  A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution.

Authors:  N Hamajima; K Matsuda; S Sakata; N Tamaki; M Sasaki; M Nonaka
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9.  Crystal structure of D-Hydantoinase from Burkholderia pickettii at a resolution of 2.7 Angstroms: insights into the molecular basis of enzyme thermostability.

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10.  Bovine liver dihydropyrimidine amidohydrolase: purification, properties, and characterization as a zinc metalloenzyme.

Authors:  K P Brooks; E A Jones; B D Kim; E G Sander
Journal:  Arch Biochem Biophys       Date:  1983-10-15       Impact factor: 4.013
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  3 in total

1.  Purification, crystallization and X-ray diffraction analysis of dihydropyrimidinase from Dictyostelium discoideum.

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Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-12-16

2.  Identification of fungal dihydrouracil-oxidase genes by expression in Saccharomyces cerevisiae.

Authors:  Jonna Bouwknegt; Aurin M Vos; Raúl A Ortiz Merino; Daphne C van Cuylenburg; Marijke A H Luttik; Jack T Pronk
Journal:  Antonie Van Leeuwenhoek       Date:  2022-10-14       Impact factor: 2.158

3.  Global expression analysis of the yeast Lachancea (Saccharomyces) kluyveri reveals new URC genes involved in pyrimidine catabolism.

Authors:  Anna Andersson Rasmussen; Dineshkumar Kandasamy; Halfdan Beck; Seth D Crosby; Olof Björnberg; Klaus D Schnackerz; Jure Piškur
Journal:  Eukaryot Cell       Date:  2013-11-01
  3 in total

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