Literature DB >> 16508108

Expression, purification and preliminary crystallographic analysis of sucrose phosphate synthase (SPS) from Halothermothrix orenii.

Frederick Huynh1, Tien-Chye Tan, Kunchithapadam Swaminathan, Bharat K C Patel.   

Abstract

This is the first report of the crystallization of a sucrose phosphate synthase (SPS; EC 2.4.1.14). It also constitutes the first study of a sucrose phosphate synthase from a non-photosynthetic thermohalophilic anaerobic bacterium, Halothermothrix orenii. The purified recombinant spsA protein has been crystallized in the monoclinic space group C2, with unit-cell parameters a = 154.2, b = 47.9, c = 72.3 A, beta = 103.16 degrees, using the hanging-drop vapour-diffusion method. The crystal diffracts X-rays to a resolution limit of 3.01 A. Heavy-metal and halide-soaking trials are currently in progress to solve the structure.

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Year:  2004        PMID： 16508108      PMCID： PMC1952387          DOI： 10.1107/S174430910403091X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  7 in total

1.  Crystallization of an alpha-amylase, AmyA, from the thermophilic halophile Halothermothrix orenii.

Authors:  Nan Li; Bharat K C Patel; Benjamin N Mijts; Kunchithapadam Swaminathan
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-11-23

2.  Crystallization of a novel alpha-amylase, AmyB, from the thermophilic halophile Halothermothrix orenii.

Authors:  Tien-Chye Tan; Yvette Y Yien; Bharat K C Patel; Benjamin N Mijts; Kunchithapadam Swaminathan
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2003-11-27

3.  Processing of X-ray diffraction data collected in oscillation mode.

Authors:  Z Otwinowski; W Minor
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

4.  Random sequence analysis of genomic DNA of an anaerobic, thermophilic, halophilic bacterium, Halothermothrix orenii.

Authors:  B N Mijts; B K Patel
Journal:  Extremophiles       Date:  2001-02       Impact factor: 2.395

5.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

6.  Elevated Levels of Both Sucrose-Phosphate Synthase and Sucrose Synthase in Vicia Guard Cells Indicate Cell-Specific Carbohydrate Interconversions.

Authors:  DRC. Hite; W. H. Outlaw; M. C. Tarczynski
Journal:  Plant Physiol       Date:  1993-04       Impact factor: 8.340

7.  Isolation and characterization of Halothermothrix orenii gen. nov., sp. nov., a halophilic, thermophilic, fermentative, strictly anaerobic bacterium.

Authors:  J L Cayol; B Ollivier; B K Patel; G Prensier; J Guezennec; J L Garcia
Journal:  Int J Syst Bacteriol       Date:  1994-07
  7 in total
  5 in total

1.  Expression, purification, crystallization and preliminary X-ray diffraction analysis of a ribokinase from the thermohalophile Halothermothrix orenii.

Authors:  Lokesh D Kori; Andreas Hofmann; Bharat K C Patel
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-01-27

2.  Expression, purification and preliminary crystallographic analysis of the recombinant β-glucosidase (BglA) from the halothermophile Halothermothrix orenii.

Authors:  Lokesh D Kori; Andreas Hofmann; Bharat K C Patel
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-12-23

3.  The structure of sucrose phosphate synthase from Halothermothrix orenii reveals its mechanism of action and binding mode.

Authors:  Teck Khiang Chua; Janusz M Bujnicki; Tien-Chye Tan; Frederick Huynh; Bharat K Patel; J Sivaraman
Journal:  Plant Cell       Date:  2008-04-18       Impact factor: 11.277

4.  Purification and characterization of recombinant sugarcane sucrose phosphate synthase expressed in E. coli and insect Sf9 cells: an importance of the N-terminal domain for an allosteric regulatory property.

Authors:  Widhi Dyah Sawitri; Hirotaka Narita; Etsuko Ishizaka-Ikeda; Bambang Sugiharto; Toshiharu Hase; Atsushi Nakagawa
Journal:  J Biochem       Date:  2016-01-30       Impact factor: 3.387

5.  Genome analysis of the anaerobic thermohalophilic bacterium Halothermothrix orenii.

Authors:  Konstantinos Mavromatis; Natalia Ivanova; Iain Anderson; Athanasios Lykidis; Sean D Hooper; Hui Sun; Victor Kunin; Alla Lapidus; Philip Hugenholtz; Bharat Patel; Nikos C Kyrpides
Journal:  PLoS One       Date:  2009-01-15       Impact factor: 3.240
  5 in total

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