Literature DB >> 16508087

Purification and crystallization of a trimodular complex comprising the type II cohesin-dockerin interaction from the cellulosome of Clostridium thermocellum.

Jarrett J Adams1, Gour Pal, Katherine Yam, Holly L Spencer, Zongchao Jia, Steven P Smith.   

Abstract

The high-affinity calcium-mediated type II cohesin-dockerin interaction is responsible for the attachment of the multi-enzyme cellulose-degrading complex, termed the cellulosome, to the cell surface of the thermophilic anaerobe Clostridium thermocellum. A trimodular 40 kDa complex comprising the SdbA type II cohesin and the the CipA type II dockerin-X module modular pair from the cellulosome of C. thermocellum has been crystallized. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 45.21, b = 52.34, c = 154.69 A. The asymmetric unit contains one molecule of the protein complex and native and selenomethionine-derivative crystals diffracted to 2.1 and 2.0 A, respectively.

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Year:  2004        PMID： 16508087      PMCID： PMC1952395          DOI： 10.1107/S1744309104025837

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  25 in total

1.  Solution structure of a type I dockerin domain, a novel prokaryotic, extracellular calcium-binding domain.

Authors:  B L Lytle; B F Volkman; W M Westler; M P Heckman; J H Wu
Journal:  J Mol Biol       Date:  2001-03-30       Impact factor: 5.469

2.  Cohesin-dockerin recognition in cellulosome assembly: experiment versus hypothesis.

Authors:  A Mechaly; S Yaron; R Lamed; H P Fierobe; A Belaich; J P Belaich; Y Shoham; E A Bayer
Journal:  Proteins       Date:  2000-05-01

3.  Mapping by site-directed mutagenesis of the region responsible for cohesin-dockerin interaction on the surface of the seventh cohesin domain of Clostridium thermocellum CipA.

Authors:  Isabelle Miras; Francis Schaeffer; Pierre Béguin; Pedro M Alzari
Journal:  Biochemistry       Date:  2002-02-19       Impact factor: 3.162

Review 4.  The cellulosome and cellulose degradation by anaerobic bacteria.

Authors:  W H Schwarz
Journal:  Appl Microbiol Biotechnol       Date:  2001-09       Impact factor: 4.813

5.  Preliminary X-ray characterization and phasing of a type II cohesin domain from the cellulosome of Acetivibrio cellulolyticus.

Authors:  Ilit Noach; Raphael Lamed; Qi Xu; Sonia Rosenheck; Linda J W Shimon; Edward A Bayer; Felix Frolow
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2003-08-19

Review 6.  Cellulosomes-structure and ultrastructure.

Authors:  E A Bayer; L J Shimon; Y Shoham; R Lamed
Journal:  J Struct Biol       Date:  1998-12-15       Impact factor: 2.867

7.  Duplicated dockerin subdomains of Clostridium thermocellum endoglucanase CelD bind to a cohesin domain of the scaffolding protein CipA with distinct thermodynamic parameters and a negative cooperativity.

Authors:  Francis Schaeffer; Markus Matuschek; Gérard Guglielmi; Isabelle Miras; Pedro M Alzari; Pierre Béguin
Journal:  Biochemistry       Date:  2002-02-19       Impact factor: 3.162

8.  Cohesin-dockerin interaction in cellulosome assembly: a single hydroxyl group of a dockerin domain distinguishes between nonrecognition and high affinity recognition.

Authors:  A Mechaly; H P Fierobe; A Belaich; J P Belaich; R Lamed; Y Shoham; E A Bayer
Journal:  J Biol Chem       Date:  2001-01-08       Impact factor: 5.157

9.  Crystal structure of a cohesin module from Clostridium cellulolyticum: implications for dockerin recognition.

Authors:  S Spinelli; H P Fiérobe; A Belaïch; J P Belaïch; B Henrissat; C Cambillau
Journal:  J Mol Biol       Date:  2000-11-24       Impact factor: 5.469

10.  1H, 13C, 15N NMR sequence-specific resonance assignment of a Clostridium thermocellum type II cohesin module.

Authors:  Steven P Smith; Pierre Béguin; Pedro M Alzari; Kalle Gehring
Journal:  J Biomol NMR       Date:  2002-05       Impact factor: 2.835
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  4 in total

1.  Mechanism of bacterial cell-surface attachment revealed by the structure of cellulosomal type II cohesin-dockerin complex.

Authors:  Jarrett J Adams; Gour Pal; Zongchao Jia; Steven P Smith
Journal:  Proc Natl Acad Sci U S A       Date:  2005-12-29       Impact factor: 11.205

Review 2.  Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives.

Authors:  Raj Kumar; Sompal Singh; Om V Singh
Journal:  J Ind Microbiol Biotechnol       Date:  2008-03-13       Impact factor: 3.346

3.  Atypical cohesin-dockerin complex responsible for cell surface attachment of cellulosomal components: binding fidelity, promiscuity, and structural buttresses.

Authors:  Orly Salama-Alber; Maroor K Jobby; Seth Chitayat; Steven P Smith; Bryan A White; Linda J W Shimon; Raphael Lamed; Felix Frolow; Edward A Bayer
Journal:  J Biol Chem       Date:  2013-04-11       Impact factor: 5.157

4.  Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family-related protein-protein interactions.

Authors:  Vered Israeli-Ruimy; Pedro Bule; Sadanari Jindou; Bareket Dassa; Sarah Moraïs; Ilya Borovok; Yoav Barak; Michal Slutzki; Yuval Hamberg; Vânia Cardoso; Victor D Alves; Shabir Najmudin; Bryan A White; Harry J Flint; Harry J Gilbert; Raphael Lamed; Carlos M G A Fontes; Edward A Bayer
Journal:  Sci Rep       Date:  2017-02-10       Impact factor: 4.379
  4 in total

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