Literature DB >> 16508086

Crystallization and preliminary crystallographic analysis of the nickel-responsive regulator NikR from Pyrococcus horikoshii.

Tomoe Kitao1, Chizu Kuroishi, Tahir H Tahirov.   

Abstract

The nickel-responsive repressor from Pyrococcus horikoshii OT3 (PhNikR) has been crystallized in the apo form (PhNikR-apo) and two nickel-bound forms (PhNikR-Ni-1 and PhNikR-Ni-2). The PhNikR-apo crystals belong to space group P2(1), with unit-cell parameters a = 75.78, b = 54.32, c = 77.28 A, beta = 116.07 degrees, and diffract to 2.2 A. The PhNikR-Ni-1 crystals belong to space group P4(1)2(1)2, with unit-cell parameters a = b = 99.89, c = 97.98 A, and diffract to 3.0 A and the PhNikR-Ni-2 crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 109.95, c = 79.0 A, and diffract to 2.1 A. The crystals obtained were suitable for detailed structural studies.

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Year:  2004        PMID： 16508086      PMCID： PMC1952384          DOI： 10.1107/S1744309104025473

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  13 in total

1.  NikR is a ribbon-helix-helix DNA-binding protein.

Authors:  P T Chivers; R T Sauer
Journal:  Protein Sci       Date:  1999-11       Impact factor: 6.725

2.  Regulation of high affinity nickel uptake in bacteria. Ni2+-Dependent interaction of NikR with wild-type and mutant operator sites.

Authors:  P T Chivers; R T Sauer
Journal:  J Biol Chem       Date:  2000-06-30       Impact factor: 5.157

Review 3.  Structure/function relationships in nickel metallobiochemistry.

Authors:  M J Maroney
Journal:  Curr Opin Chem Biol       Date:  1999-04       Impact factor: 8.822

4.  Screening and optimization strategies for macromolecular crystal growth.

Authors:  R Cudney; S Patel; K Weisgraber; Y Newhouse; A McPherson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-07-01

5.  Crystal structure of the nickel-responsive transcription factor NikR.

Authors:  Eric R Schreiter; Michael D Sintchak; Yayi Guo; Peter T Chivers; Robert T Sauer; Catherine L Drennan
Journal:  Nat Struct Biol       Date:  2003-09-14

6.  Processing of X-ray diffraction data collected in oscillation mode.

Authors:  Z Otwinowski; W Minor
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

7.  NikR repressor: high-affinity nickel binding to the C-terminal domain regulates binding to operator DNA.

Authors:  Peter T Chivers; Robert T Sauer
Journal:  Chem Biol       Date:  2002-10

Review 8.  Nickel transport systems in microorganisms.

Authors:  T Eitinger; M A Mandrand-Berthelot
Journal:  Arch Microbiol       Date:  2000-01       Impact factor: 2.552

9.  Structure and implications for the thermal stability of phosphopantetheine adenylyltransferase from Thermus thermophilus.

Authors:  Hitomi Takahashi; Eiji Inagaki; Yayoi Fujimoto; Chizu Kuroishi; Yuichi Nodake; Yuki Nakamura; Fumio Arisaka; Katsuhide Yutani; Seiki Kuramitsu; Shigeyuki Yokoyama; Masaki Yamamoto; Masashi Miyano; Tahir H Tahirov
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2003-12-18

10.  Nickel coordination is regulated by the DNA-bound state of NikR.

Authors:  Paul E Carrington; Peter T Chivers; Faizah Al-Mjeni; Robert T Sauer; Michael J Maroney
Journal:  Nat Struct Biol       Date:  2003-02
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  1 in total

1.  Physical basis of metal-binding specificity in Escherichia coli NikR.

Authors:  Christine M Phillips; Paul S Nerenberg; Catherine L Drennan; Collin M Stultz
Journal:  J Am Chem Soc       Date:  2009-07-29       Impact factor: 15.419
  1 in total

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