| Literature DB >> 1649702 |
R Klein1, V Nanduri, S A Jing, F Lamballe, P Tapley, S Bryant, C Cordon-Cardo, K R Jones, L F Reichardt, M Barbacid.
Abstract
trkB is a tyrosine protein kinase gene highly related to trk, a proto-oncogene that encodes a receptor for nerve growth factor (NGF) and neurotrophin-3 (NT-3). trkB expression is confined to structures of the central and peripheral nervous systems, suggesting it also encodes a receptor for neurotrophic factors. Here we show that brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of gp145trkB, one of the receptors encoded by trkB. BDNF and NT-3 can induce DNA synthesis in quiescent NIH 3T3 cells that express gp145trkB. Cotransfection of plasmids encoding gp145trkB and BDNF or NT-3 leads to transformation of recipient NIH 3T3 cells. In these assays, BDNF elicits a response at least two orders of magnitude higher than NT-3. Finally, 125I-NT-3 binds to NIH 3T3 cells expressing gp145trkB; binding can be competed by NT-3 and BDNF but not by NGF. These findings indicate that gp145trkB may function as a neurotrophic receptor for BDNF and NT-3.Entities:
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Year: 1991 PMID： 1649702 PMCID： PMC2710095 DOI： 10.1016/0092-8674(91)90628-c
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582