BACKGROUND: Gene regulated in breast cancer 1 (GREB1) is a novel estrogen-regulated gene shown to play a pivotal role in hormone-stimulated breast cancer growth. GREB1 is expressed in the prostate and its putative promoter contains potential androgen receptor (AR) response elements. METHODS: We investigated the effects of androgens on GREB1 expression and its role in androgen-dependent prostate cancer growth. RESULTS: Real-time PCR demonstrated high level GREB1 expression in benign prostatic hypertrophy (BPH), localized prostate cancer (L-PCa), and hormone refractory prostate cancer (HR-PCa). Androgen treatment of AR-positive prostate cancer cells induced dose-dependent GREB1 expression, which was blocked by anti-androgens. AR binding to the GREB1 promoter was confirmed by chromatin immunoprecipitation (ChIP) assays. Suppression of GREB1 by RNA interference blocked androgen-stimulated LNCaP cell proliferation. CONCLUSIONS: GREB1 is expressed in proliferating prostatic tissue and prostate cancer, is regulated by androgens, and suppression of GREB1 blocks androgen-induced growth suggesting GREB1 may be critically involved in prostate cancer proliferation.
BACKGROUND: Gene regulated in breast cancer 1 (GREB1) is a novel estrogen-regulated gene shown to play a pivotal role in hormone-stimulated breast cancer growth. GREB1 is expressed in the prostate and its putative promoter contains potential androgen receptor (AR) response elements. METHODS: We investigated the effects of androgens on GREB1 expression and its role in androgen-dependent prostate cancer growth. RESULTS: Real-time PCR demonstrated high level GREB1 expression in benign prostatic hypertrophy (BPH), localized prostate cancer (L-PCa), and hormone refractory prostate cancer (HR-PCa). Androgen treatment of AR-positive prostate cancer cells induced dose-dependent GREB1 expression, which was blocked by anti-androgens. AR binding to the GREB1 promoter was confirmed by chromatin immunoprecipitation (ChIP) assays. Suppression of GREB1 by RNA interference blocked androgen-stimulated LNCaP cell proliferation. CONCLUSIONS:GREB1 is expressed in proliferating prostatic tissue and prostate cancer, is regulated by androgens, and suppression of GREB1 blocks androgen-induced growth suggesting GREB1 may be critically involved in prostate cancer proliferation.
Authors: Patrick D Brophy; Maria Rasmussen; Mrutyunjaya Parida; Greg Bonde; Benjamin W Darbro; Xiaojing Hong; Jason C Clarke; Kevin A Peterson; James Denegre; Michael Schneider; Caroline R Sussman; Lone Sunde; Dorte L Lildballe; Jens Michael Hertz; Robert A Cornell; Stephen A Murray; J Robert Manak Journal: Genetics Date: 2017-07-24 Impact factor: 4.562
Authors: Alison J Camden; Maria M Szwarc; Sangappa B Chadchan; Francesco J DeMayo; Bert W O'Malley; John P Lydon; Ramakrishna Kommagani Journal: Mol Hum Reprod Date: 2017-09-01 Impact factor: 4.025
Authors: Rainer B Lanz; Yaroslava Bulynko; Anna Malovannaya; Paul Labhart; Liguo Wang; Wei Li; Jun Qin; Mary Harper; Bert W O'Malley Journal: Mol Endocrinol Date: 2010-02-24
Authors: Kevin G Hegarty; Frances J Drummond; Mary Daly; Fergus Shanahan; Michael G Molloy Journal: J Bone Miner Metab Date: 2017-03-14 Impact factor: 2.626
Authors: Teresa D Gallardo; George B John; Lane Shirley; Cristina M Contreras; Esra A Akbay; J Marshall Haynie; Samuel E Ward; Meredith J Shidler; Diego H Castrillon Journal: Genetics Date: 2007-07-29 Impact factor: 4.562
Authors: Hisham Mohammed; Clive D'Santos; Aurelien A Serandour; H Raza Ali; Gordon D Brown; Alan Atkins; Oscar M Rueda; Kelly A Holmes; Vasiliki Theodorou; Jessica L L Robinson; Wilbert Zwart; Amel Saadi; Caryn S Ross-Innes; Suet-Feung Chin; Suraj Menon; John Stingl; Carlo Palmieri; Carlos Caldas; Jason S Carroll Journal: Cell Rep Date: 2013-02-09 Impact factor: 9.423