Literature DB >> 16494209

[Comparision of the sensitivity of detection of bacteria in human blood using classic culture methods and molecular techniques: PCR and FISH].

Tomasz Gosiewski1, Agnieszka Kasprzyk, Magdalena Strus.   

Abstract

Blood infections are one of the major problems of the contemporary medical care. Their detection depends strongly on sensitivity of the used microbiological methods. Widely applied classical techniques based on blood cultivation are time consuming and less sensitive. Therefore, it is necessary to search for new molecular tools to detect bacteria in blood directly taken from patient, like as PCR or FISH. In this paper we have proved that both PCR and FISH show very high sensitivity but due to various limitations their procedures need for further standardization before future application to routine diagnosis of blood infections.

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Year:  2005        PMID: 16494209

Source DB:  PubMed          Journal:  Med Dosw Mikrobiol        ISSN: 0025-8601


  3 in total

1.  The application of genetics methods to differentiation of three Lactobacillus species of human origin.

Authors:  Tomasz Gosiewski; Agnieszka Chmielarczyk; Magdalena Strus; Monika Brzychczy-Włoch; Piotr B Heczko
Journal:  Ann Microbiol       Date:  2011-12-10       Impact factor: 2.112

2.  Comparison of PCR, Fluorescent in Situ Hybridization and Blood Cultures for Detection of Bacteremia in Children and Adolescents During Antibiotic Therapy.

Authors:  Tomasz W Źródłowski; Danuta Jurkiewicz-Badacz; Agnieszka Sroka-Oleksiak; Dominika Salamon; Małgorzata Bulanda; Tomasz Gosiewski
Journal:  Pol J Microbiol       Date:  2018

3.  Evaluation of FISH for Blood Cultures under Diagnostic Real-Life Conditions.

Authors:  Annalena Reitz; Sven Poppert; Melanie Rieker; Hagen Frickmann
Journal:  Eur J Microbiol Immunol (Bp)       Date:  2018-12-12
  3 in total

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