Literature DB >> 1649335

Analysis of the herpes simplex virus type 1 OriS sequence: mapping of functional domains.

D W Martin1, S P Deb, J S Klauer, S Deb.   

Abstract

The herpes simplex virus type 1 (HSV-1) OriS region resides within a 90-bp sequence that contains two binding sites for the origin-binding protein (OBP), designated sites I and II. A third presumptive OBP-binding site (III) within OriS has strong sequence similarity to sites I and II, but no sequence-specific OBP binding has yet been demonstrated at this site. We have generated mutations in sites I, II, and III and determined their replication efficiencies in a transient in vivo assay in the presence of a helper virus. Mutations in any one of the sites reduced DNA replication significantly. To study the role of OriS sequence elements in site I and the presumptive site III in DNA replication, we have also generated a series of mutations that span from site I across the presumptive binding site III. These mutants were tested for their ability to replicate and for the ability to bind OBP by using gel shift analyses. The results indicate that mutations across site I drastically reduce DNA replication. Triple-base-pair substitution mutations that fall within the crucial OBP-binding domain, 5'-YGYTCGCACT-3' (where Y represents C or T), show a reduced level of OBP binding and DNA replication. Substitution mutations in site I that are outside this crucial binding sequence show a more detrimental effect on DNA replication than on OBP binding. This suggests that these sequences are required for initiation of DNA replication but are not critical for OBP binding. Mutations across the presumptive OBP-binding site III also resulted in a loss in efficiency of DNA replication. These mutations influenced OBP binding to OriS in gel shift assays, even though the mutated sequences are not contained within known OBP-binding sites. Replacement of the wild-type site III with a perfect OBP-binding site I results in a drastic reduction of DNA replication. Thus, our DNA replication assays and in vitro DNA-binding studies suggest that the binding of the origin sequence by OBP is not the only determining factor for initiation of DNA replication in vivo.

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Year:  1991        PMID: 1649335      PMCID: PMC248875     

Source DB:  PubMed          Journal:  J Virol        ISSN: 0022-538X            Impact factor:   5.103


  31 in total

1.  Identification of herpes simplex virus type 1 genes required for origin-dependent DNA synthesis.

Authors:  C A Wu; N J Nelson; D J McGeoch; M D Challberg
Journal:  J Virol       Date:  1988-02       Impact factor: 5.103

2.  A 269-amino-acid segment with a pseudo-leucine zipper and a helix-turn-helix motif codes for the sequence-specific DNA-binding domain of herpes simplex virus type 1 origin-binding protein.

Authors:  S Deb; S P Deb
Journal:  J Virol       Date:  1991-06       Impact factor: 5.103

3.  Sequence and structural requirements of a herpes simplex viral DNA replication origin.

Authors:  D Lockshon; D A Galloway
Journal:  Mol Cell Biol       Date:  1988-10       Impact factor: 4.272

4.  Herpes simplex virus DNA replication: the UL9 gene encodes an origin-binding protein.

Authors:  P D Olivo; N J Nelson; M D Challberg
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

5.  Characterization of major recognition sequences for a herpes simplex virus type 1 origin-binding protein.

Authors:  A Koff; P Tegtmeyer
Journal:  J Virol       Date:  1988-11       Impact factor: 5.103

6.  Structures of herpes simplex virus type 1 genes required for replication of virus DNA.

Authors:  D J McGeoch; M A Dalrymple; A Dolan; D McNab; L J Perry; P Taylor; M D Challberg
Journal:  J Virol       Date:  1988-02       Impact factor: 5.103

7.  A 67-base-pair segment from the Ori-S region of herpes simplex virus type 1 encodes origin function.

Authors:  S Deb; M Doelberg
Journal:  J Virol       Date:  1988-07       Impact factor: 5.103

8.  The origin binding protein of herpes simplex virus 1 binds cooperatively to the viral origin of replication oris.

Authors:  P Elias; C M Gustafsson; O Hammarsten
Journal:  J Biol Chem       Date:  1990-10-05       Impact factor: 5.157

Review 9.  The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1.

Authors:  D J McGeoch; M A Dalrymple; A J Davison; A Dolan; M C Frame; D McNab; L J Perry; J E Scott; P Taylor
Journal:  J Gen Virol       Date:  1988-07       Impact factor: 3.891

10.  Interaction of origin binding protein with an origin of replication of herpes simplex virus 1.

Authors:  P Elias; I R Lehman
Journal:  Proc Natl Acad Sci U S A       Date:  1988-05       Impact factor: 11.205

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  20 in total

1.  A sequence within the varicella-zoster virus (VZV) OriS is a negative regulator of DNA replication and is bound by a protein complex containing the VZV ORF29 protein.

Authors:  Mohamed I Khalil; Ann Arvin; Jeremy Jones; William T Ruyechan
Journal:  J Virol       Date:  2011-09-21       Impact factor: 5.103

2.  Purification and characterization of UL9, the herpes simplex virus type 1 origin-binding protein.

Authors:  D S Fierer; M D Challberg
Journal:  J Virol       Date:  1992-07       Impact factor: 5.103

3.  Properties of the novel herpes simplex virus type 1 origin binding protein, OBPC.

Authors:  K Baradaran; M A Hardwicke; C E Dabrowski; P A Schaffer
Journal:  J Virol       Date:  1996-08       Impact factor: 5.103

4.  Cellular transcription factors enhance herpes simplex virus type 1 oriS-dependent DNA replication.

Authors:  A T Nguyen-Huynh; P A Schaffer
Journal:  J Virol       Date:  1998-05       Impact factor: 5.103

5.  An Sp1/Sp3 site in the downstream region of varicella-zoster virus (VZV) oriS influences origin-dependent DNA replication and flanking gene transcription and is important for VZV replication in vitro and in human skin.

Authors:  Mohamed I Khalil; Makeda Robinson; Marvin Sommer; Ann Arvin; John Hay; William T Ruyechan
Journal:  J Virol       Date:  2012-08-29       Impact factor: 5.103

6.  Cloning and expression of an equine herpesvirus 1 origin-binding protein.

Authors:  D W Martin; S Deb
Journal:  J Virol       Date:  1994-06       Impact factor: 5.103

7.  Transcriptional analysis of the region of the herpes simplex virus type 1 genome containing the UL8, UL9, and UL10 genes and identification of a novel delayed-early gene product, OBPC.

Authors:  K Baradaran; C E Dabrowski; P A Schaffer
Journal:  J Virol       Date:  1994-07       Impact factor: 5.103

8.  Stepwise evolution of the herpes simplex virus origin binding protein and origin of replication.

Authors:  Monica Olsson; Ka-Wei Tang; Cecilia Persson; L Marcus Wilhelmsson; Martin Billeter; Per Elias
Journal:  J Biol Chem       Date:  2009-04-07       Impact factor: 5.157

9.  Human herpesvirus 6B origin: sequence diversity, requirement for two binding sites for origin-binding protein, and enhanced replication from origin multimers.

Authors:  S Dewhurst; D M Krenitsky; C Dykes
Journal:  J Virol       Date:  1994-10       Impact factor: 5.103

10.  Complete sequence and comparative analysis of the genome of herpes B virus (Cercopithecine herpesvirus 1) from a rhesus monkey.

Authors:  Ludmila Perelygina; Li Zhu; Holley Zurkuhlen; Ryan Mills; Mark Borodovsky; Julia K Hilliard
Journal:  J Virol       Date:  2003-06       Impact factor: 5.103

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