Pulsed electric field extraction of polysaccharide from Rana temporaria chensinensis David.

In order to develop and optimize a pulsed electric field (PEF) extraction method and evaluate it against conventional extraction methods for the extraction of polysaccharide from Rana temporaria chensinensis David, we have investigated various experimental conditions, respectively, such as electric field intensity (10-30 kV/cm), pulse duration (2-6 micros) and concentration of distilling solvent (0-1% KOH, v/v), and then optimized them by an orthogonal experiment. The result showed that the largest extraction ratio is 55.59% by PEF on the conditions of 0.5% KOH, 20 kV/cm electric field intensity and 6 micros pulse duration. Comparing it with the conventional extraction methods, such as alkali extraction method, enzyme extraction method and compound extraction method, the extraction ratio and polysaccharide content of PEF method are higher than the other three methods. The PEF extraction ratio for 6 micros is 1.77 times the compound extraction method for 6 h, the total sugar contents are more than 26.34% of that for the compound extraction method and the impurity of extraction material is less. So the PEF method is a novel and promising method to extract polysaccharide of R. temporaria chensinensis David.