Literature DB >> 1648108

Production, isolation and characterization of human profilin from Saccharomyces cerevisiae.

P Aspenström1, I Lassing, R Karlsson.   

Abstract

The yeast Saccharomyces cerevisiae was used to express human profilin cDNA. The recombinant protein, isolated by affinity chromatography on poly(L-proline)-Sepharose followed by ion exchange chromatography, associates with non-muscle actin and phosphatidylinositol-(4,5)-bisphosphate as authentic profilin.

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Year:  1991        PMID： 1648108     DOI： 10.1007/bf01774039

Source DB:  PubMed          Journal:  J Muscle Res Cell Motil        ISSN： 0142-4319            Impact factor:   2.698


  30 in total

1.  Evidence that the phosphatidylinositol cycle is linked to cell motility.

Authors:  I Lassing; U Lindberg
Journal:  Exp Cell Res       Date:  1988-01       Impact factor: 3.905

2.  The effect of divalent cations on the interaction between calf spleen profilin and different actins.

Authors:  H Larsson; U Lindberg
Journal:  Biochim Biophys Acta       Date:  1988-03-02

3.  The intron-containing gene for yeast profilin (PFY) encodes a vital function.

Authors:  V Magdolen; U Oechsner; G Müller; W Bandlow
Journal:  Mol Cell Biol       Date:  1988-12       Impact factor: 4.272

4.  The amino acid sequence of Acanthamoeba profilin.

Authors:  C Ampe; J Vandekerckhove; S L Brenner; L Tobacman; E D Korn
Journal:  J Biol Chem       Date:  1985-01-25       Impact factor: 5.157

5.  Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I.

Authors:  I Blikstad; F Markey; L Carlsson; T Persson; U Lindberg
Journal:  Cell       Date:  1978-11       Impact factor: 41.582

6.  Human profilin. Molecular cloning, sequence comparison, and chromosomal analysis.

Authors:  D J Kwiatkowski; G A Bruns
Journal:  J Biol Chem       Date:  1988-04-25       Impact factor: 5.157

7.  The actin-binding protein profilin binds to PIP2 and inhibits its hydrolysis by phospholipase C.

Authors:  P J Goldschmidt-Clermont; L M Machesky; J J Baldassare; T D Pollard
Journal:  Science       Date:  1990-03-30       Impact factor: 47.728

8.  The profilin--actin complex: further characterization of profilin and studies on the stability of the complex.

Authors:  B Malm; H Larsson; U Lindberg
Journal:  J Muscle Res Cell Motil       Date:  1983-10       Impact factor: 2.698

9.  Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.

Authors:  R Ng; J Abelson
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

10.  Mechanism of action of Acanthamoeba profilin: demonstration of actin species specificity and regulation by micromolar concentrations of MgCl2.

Authors:  P C Tseng; T D Pollard
Journal:  J Cell Biol       Date:  1982-07       Impact factor: 10.539
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