OBJECTIVE: To evaluate bacterial and protozoal contamination of commercially available raw meat diets for dogs. DESIGN: Prospective longitudinal study. SAMPLE POPULATION: 240 samples from 20 raw meat diets for dogs (containing beef, lamb, chicken, or turkey), 24 samples from 2 dry dog foods, and 24 samples from 2 canned dog foods. PROCEDURE: Each product was purchased commercially on 4 dates approximately 2 months apart. Three samples from each product at each sampling period were evaluated via bacterial culture for non-type-specific Escherichia coli (NTSEC), Salmonella enterica, and Campylobacter spp. Antimicrobial susceptibility testing was performed on selected isolates. Polymerase chain reaction assays were used to detect DNA from Cryptosporidium spp, Neospora spp, and Toxoplasma spp in samples obtained in the third and fourth sampling periods. RESULTS: One hundred fifty-three of 288 (53%) samples were contaminated with NTSEC. Both raw and prepared foods contained NTSEC during at least 1 culture period. Salmonella enterica was recovered from 17 (5.9%) samples, all of which were raw meat products. Campylobacter spp was not isolated from any samples. In 91 of 288 (31.6%) samples, there was no gram-negative bacterial growth before enrichment and in 48 of 288 (16.7%) samples, there was no aerobic bacterial growth before enrichment. Susceptibility phenotypes were variable. Cryptosporidium spp DNA was detected in 3 samples. CONCLUSIONS AND CLINICAL RELEVANCE: Bacterial contamination is common in commercially available raw meat diets, suggesting that there is a risk of foodborne illness in dogs fed these diets as well possible risk for humans associated with the dogs or their environments.
OBJECTIVE: To evaluate bacterial and protozoal contamination of commercially available raw meat diets for dogs. DESIGN: Prospective longitudinal study. SAMPLE POPULATION: 240 samples from 20 raw meat diets for dogs (containing beef, lamb, chicken, or turkey), 24 samples from 2 dry dog foods, and 24 samples from 2 canned dog foods. PROCEDURE: Each product was purchased commercially on 4 dates approximately 2 months apart. Three samples from each product at each sampling period were evaluated via bacterial culture for non-type-specific Escherichia coli (NTSEC), Salmonella enterica, and Campylobacter spp. Antimicrobial susceptibility testing was performed on selected isolates. Polymerase chain reaction assays were used to detect DNA from Cryptosporidium spp, Neospora spp, and Toxoplasma spp in samples obtained in the third and fourth sampling periods. RESULTS: One hundred fifty-three of 288 (53%) samples were contaminated with NTSEC. Both raw and prepared foods contained NTSEC during at least 1 culture period. Salmonella enterica was recovered from 17 (5.9%) samples, all of which were raw meat products. Campylobacter spp was not isolated from any samples. In 91 of 288 (31.6%) samples, there was no gram-negative bacterial growth before enrichment and in 48 of 288 (16.7%) samples, there was no aerobic bacterial growth before enrichment. Susceptibility phenotypes were variable. Cryptosporidium spp DNA was detected in 3 samples. CONCLUSIONS AND CLINICAL RELEVANCE: Bacterial contamination is common in commercially available raw meat diets, suggesting that there is a risk of foodborne illness in dogs fed these diets as well possible risk for humans associated with the dogs or their environments.
Authors: Lieve Lucia Meers; Laura Contalbrigo; William Ellery Samuels; Carolina Duarte-Gan; Daniel Berckmans; Stephan Jens Laufer; Vicky Antoinette Stevens; Elizabeth Ann Walsh; Simona Normando Journal: Front Vet Sci Date: 2022-06-17
Authors: Satu Olkkola; Sara Kovanen; Johanna Roine; Marja-Liisa Hänninen; Anna Hielm-Björkman; Rauni Kivistö Journal: PLoS One Date: 2015-07-14 Impact factor: 3.240
Authors: Renate Reimschuessel; Michael Grabenstein; Jake Guag; Sarah M Nemser; Kyunghee Song; Junshan Qiu; Kristin A Clothier; Barbara A Byrne; Stanley L Marks; Kyran Cadmus; Kristy Pabilonia; Susan Sanchez; Sreekumari Rajeev; Steve Ensley; Timothy S Frana; Albert E Jergens; Kimberly H Chappell; Siddhartha Thakur; Beverly Byrum; Jing Cui; Yan Zhang; Matthew M Erdman; Shelley C Rankin; Russell Daly; Seema Das; Laura Ruesch; Sara D Lawhon; Shuping Zhang; Timothy Baszler; Dubraska Diaz-Campos; Faye Hartmann; Ogi Okwumabua Journal: J Clin Microbiol Date: 2017-02-15 Impact factor: 5.948