PURPOSE: In the present study two medicinal herbs Linum persicum and Euphorbia cheiradenia that are native to Iran were tested for their possible anticancer effect and induction of apoptosis on human tumor cell lines including leukemia cell lines. METHODS: The effect of methanolic extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorimetric assay. K562 and Jurkat cell lines treated with the extracts were analyzed for the induction of apoptosis by flow cytometry using propidium iodide staining. DNA fragmentation analysis was performed. RESULTS: Various concentrations of L. persicum and E. cheiradenia showed inhibitory effects on different cell lines. Two leukemic lines including K562 and Jurkat were the most sensitive cells for L. persicum with IC50 of 0.1 and 10 mug/ml, respectively. In the cultures of tumor cell lines treated with E. cheiradenia, the main inhibitory effects was for Jurkat cells with IC50 of 12.5 microg/ml. Results indicated a dose-dependent accumulation of cells in the sub-G1 phase. Study of internucleosomal DNA fragmentation showed a typical DNA laddering in agarose gels for both extracts. CONCLUSION: The present study showed cytotoxic activity of both herbs on tumor cell lines and suggests that this effect may in part be due to the induction of apoptosis in leukemic cells.
PURPOSE: In the present study two medicinal herbs Linum persicum and Euphorbia cheiradenia that are native to Iran were tested for their possible anticancer effect and induction of apoptosis on humantumor cell lines including leukemia cell lines. METHODS: The effect of methanolic extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorimetric assay. K562 and Jurkat cell lines treated with the extracts were analyzed for the induction of apoptosis by flow cytometry using propidium iodide staining. DNA fragmentation analysis was performed. RESULTS: Various concentrations of L. persicum and E. cheiradenia showed inhibitory effects on different cell lines. Two leukemic lines including K562 and Jurkat were the most sensitive cells for L. persicum with IC50 of 0.1 and 10 mug/ml, respectively. In the cultures of tumor cell lines treated with E. cheiradenia, the main inhibitory effects was for Jurkat cells with IC50 of 12.5 microg/ml. Results indicated a dose-dependent accumulation of cells in the sub-G1 phase. Study of internucleosomal DNA fragmentation showed a typical DNA laddering in agarose gels for both extracts. CONCLUSION: The present study showed cytotoxic activity of both herbs on tumor cell lines and suggests that this effect may in part be due to the induction of apoptosis in leukemic cells.
Authors: Steven M Ogbourne; Andreas Suhrbier; Brad Jones; Sarah-Jane Cozzi; Glen M Boyle; Melanie Morris; Devi McAlpine; Jenny Johns; Tania M Scott; Kirsty P Sutherland; Joy M Gardner; Thuy T T Le; Aleksandra Lenarczyk; James H Aylward; Peter G Parsons Journal: Cancer Res Date: 2004-04-15 Impact factor: 12.701
Authors: Cláudia Valente; Madalena Pedro; Aida Duarte; Maria São José Nascimento; Pedro M Abreu; Maria-José U Ferreira Journal: J Nat Prod Date: 2004-05 Impact factor: 4.050
Authors: Lilhian Alves de Araújo; Fátima Mrué; Roberpaulo Anacleto Neves; Maxley Martins Alves; Nelson Jorge da Silva-Júnior; Marcelo Seixo de Brito Silva; Paulo Roberto de Melo-Reis Journal: Arq Bras Cir Dig Date: 2015 Nov-Dec