Literature DB >> 16473625

Assay and functional properties of Rabkinesin-6/Rab6-KIFL/MKlp2 in cytokinesis.

Rüdiger Neef, Ulrike Grüneberg, Francis A Barr.   

Abstract

Here we describe methods for the characterization of the kinesin-9 family motor protein Rabkinesin-6/Rab6-KIFL/MKlp2 in cytokinesis. Here we outline biochemical assays for studying the interaction of Rabkinesin-6/Rab6-KIFL/MKlp2 with microtubules, and its regulation and interaction with the mitotic polo-like kinase 1 using recombinant proteins expressed in and purified from insect cells. Protocols for the in vivo functional analysis of Rabkinesin-6/Rab6-KIFL/MKlp2 using depletion with small interfering RNA duplexes are described.

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Year:  2005        PMID： 16473625     DOI： 10.1016/S0076-6879(05)03054-5

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  12 in total

1.  The Nup107-160 nucleoporin complex promotes mitotic events via control of the localization state of the chromosome passenger complex.

Authors:  Melpomeni Platani; Rachel Santarella-Mellwig; Markus Posch; Rudolf Walczak; Jason R Swedlow; Iain W Mattaj
Journal:  Mol Biol Cell       Date:  2009-12       Impact factor: 4.138

2.  Identification of radiation-induced expression changes in nonimmortalized human T cells.

Authors:  Era L Pogosova-Agadjanyan; Wenhong Fan; George E Georges; Jeffrey L Schwartz; Crystal M Kepler; Hana Lee; Amanda L Suchanek; Michelle R Cronk; Ariel Brumbaugh; Julia H Engel; Michi Yukawa; Lue P Zhao; Shelly Heimfeld; Derek L Stirewalt
Journal:  Radiat Res       Date:  2010-11-17       Impact factor: 2.841

3.  A new role for Rab GTPases during early mitotic stages.

Authors:  Sanchaita Das; Heidi Hehnly; Stephen Doxsey
Journal:  Small GTPases       Date:  2014-06-12

4.  Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors.

Authors:  Shin-ichiro Yoshimura; Andreas Gerondopoulos; Andrea Linford; Daniel J Rigden; Francis A Barr
Journal:  J Cell Biol       Date:  2010-10-11       Impact factor: 10.539

5.  Deregulation of Rab and Rab effector genes in bladder cancer.

Authors:  Joel R Ho; Elodie Chapeaublanc; Lisa Kirkwood; Remy Nicolle; Simone Benhamou; Thierry Lebret; Yves Allory; Jennifer Southgate; François Radvanyi; Bruno Goud
Journal:  PLoS One       Date:  2012-06-19       Impact factor: 3.240

6.  Paclitaxel targets FOXM1 to regulate KIF20A in mitotic catastrophe and breast cancer paclitaxel resistance.

Authors:  P Khongkow; A R Gomes; C Gong; E P S Man; J W-H Tsang; F Zhao; L J Monteiro; R C Coombes; R H Medema; U S Khoo; E W-F Lam
Journal:  Oncogene       Date:  2015-05-11       Impact factor: 9.867

7.  Positive expression of KIF20A indicates poor prognosis of glioma patients.

Authors:  Jia Duan; Wei Huang; Haiping Shi
Journal:  Onco Targets Ther       Date:  2016-11-02       Impact factor: 4.147

8.  Upregulation of KIF20A correlates with poor prognosis in gastric cancer.

Authors:  Yi Sheng; Wei Wang; Bo Hong; Xingwang Jiang; Ruochuan Sun; Qiang Yan; Shangxin Zhang; Mingdian Lu; Shengyi Wang; Zhen Zhang; Wenchu Lin; Yongxiang Li
Journal:  Cancer Manag Res       Date:  2018-11-23       Impact factor: 3.989

9.  KIF20A Affects the Prognosis of Bladder Cancer by Promoting the Proliferation and Metastasis of Bladder Cancer Cells.

Authors:  Tianyu Shen; Long Yang; Zheng Zhang; Jianpeng Yu; Liang Dai; Minjun Gao; Zhiqun Shang; Yuanjie Niu
Journal:  Dis Markers       Date:  2019-04-09       Impact factor: 3.434

10.  Uncovering missed indels by leveraging unmapped reads.

Authors:  Mohammad Shabbir Hasan; Xiaowei Wu; Liqing Zhang
Journal:  Sci Rep       Date:  2019-07-31       Impact factor: 4.379
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