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Literature DB >> 16472808

Sequence-specific interference by small RNAs derived from adenovirus VAI RNA.

Masayuki Sano¹, Yoshio Kato, Kazunari Taira.

Abstract

A virus-associated RNA (VAI) of adenoviruses is a cytoplasmic non-coding RNA and it plays an important role for viral replication in infected cells. VAI RNA transcripts, produced by RNA polymerase III (pol III), form tightly structured stems, which confer resistance to cellular defense systems. We demonstrate here that small RNAs of approximately 22 nucleotides are produced from a terminal stem region but not from an apical stem of VAI RNA. We determined the processing sites of VAI RNA by S1 nuclease mapping and further confirmed that the processed small RNA can act as small interfering RNAs (siRNAs) or as microRNAs (miRNAs) in transient transfection assays and during viral infection. Our data demonstrate that non-coding RNAs synthesized by pol III can be substrates for Dicer, and diced small RNAs might regulate cellular phenomena as siRNAs and miRNAs.

Entities: Disease

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Year: 2006 PMID： 16472808 DOI： 10.1016/j.febslet.2006.01.085

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

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Cited

45 in total

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Review 2. EBV Noncoding RNAs.

Authors: Rebecca L Skalsky; Bryan R Cullen
Journal: Curr Top Microbiol Immunol Date: 2015 Impact factor: 4.291

3. Innate immune response induced by baculovirus attenuates transgene expression in mammalian cells.

Authors: Chikako Ono; Akinori Ninomiya; Satomi Yamamoto; Takayuki Abe; Xiauyu Wen; Takasuke Fukuhara; Miwa Sasai; Masahiro Yamamoto; Tatsuya Saitoh; Takashi Satoh; Taro Kawai; Ken J Ishii; Shizuo Akira; Toru Okamoto; Yoshiharu Matsuura
Journal: J Virol Date: 2013-12-11 Impact factor: 5.103

10. The 5'-end heterogeneity of adenovirus virus-associated RNAI contributes to the asymmetric guide strand incorporation into the RNA-induced silencing complex.

Authors: Ning Xu; Sofia Gkountela; Khalid Saeed; Göran Akusjärvi
Journal: Nucleic Acids Res Date: 2009-09-15 Impact factor: 16.971

Sequence-specific interference by small RNAs derived from adenovirus VAI RNA.

Review 1. Noncoding RNPs of viral origin.

Review 2. EBV Noncoding RNAs.

3. Innate immune response induced by baculovirus attenuates transgene expression in mammalian cells.

Review 4. Identification and function of human cytomegalovirus microRNAs.

Review 5. The role of RNAi and microRNAs in animal virus replication and antiviral immunity.

Review 6. Viral and cellular microRNAs as determinants of viral pathogenesis and immunity.

Review 7. The roles of microRNAs in mammalian virus infection.

Review 8. Viruses, microRNAs, and host interactions.

9. Persistently adenovirus-infected lymphoid cells express microRNAs derived from the viral VAI and especially VAII RNA.

10. The 5'-end heterogeneity of adenovirus virus-associated RNAI contributes to the asymmetric guide strand incorporation into the RNA-induced silencing complex.