Literature DB >> 16472073

A nonradioactive assay for poly(a)-specific ribonuclease activity by methylene blue colorimetry.

Yuan Cheng1, Wei-Feng Liu, Yong-Bin Yan, Hai-Meng Zhou.   

Abstract

A simple nonradioactive assay, which was based on the specific shift of the absorbance maximum of methylene blue induced by its intercalation into poly(A) molecules, was developed for poly(A)-specific ribonuclease (PARN). A good linear relationship was found between the absorbance at 662 nm and the poly(A) concentration. The assay conditions, including the concentration of methylene blue, the incubation temperature and time, and the poly(A) concentration were evaluated and optimized.

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Year:  2006        PMID： 16472073     DOI： 10.2174/092986606775101580

Source DB:  PubMed          Journal:  Protein Pept Lett        ISSN： 0929-8665            Impact factor:   1.890


  9 in total

1.  AtHESPERIN: a novel regulator of circadian rhythms with poly(A)-degrading activity in plants.

Authors:  Costas Delis; Afrodite Krokida; Anastasia Tomatsidou; Daniela Tsikou; Rafailia A A Beta; Maria Tsioumpekou; Julietta Moustaka; Georgios Stravodimos; Demetres D Leonidas; Nikolaos A A Balatsos; Kalliope K Papadopoulou
Journal:  RNA Biol       Date:  2016       Impact factor: 4.652

2.  An integrated in silico approach to design specific inhibitors targeting human poly(a)-specific ribonuclease.

Authors:  Dimitrios Vlachakis; Athanasia Pavlopoulou; Georgia Tsiliki; Dimitri Komiotis; Constantinos Stathopoulos; Nikolaos A A Balatsos; Sophia Kossida
Journal:  PLoS One       Date:  2012-12-06       Impact factor: 3.240

Review 3.  Dissimilar roles of the four conserved acidic residues in the thermal stability of poly(A)-specific ribonuclease.

Authors:  Guang-Jun He; Wei-Feng Liu; Yong-Bin Yan
Journal:  Int J Mol Sci       Date:  2011-05-03       Impact factor: 5.923

4.  A deadenylase assay by size-exclusion chromatography.

Authors:  Guang-Jun He; Yong-Bin Yan
Journal:  PLoS One       Date:  2012-03-19       Impact factor: 3.240

5.  A fluorescence-based assay suitable for quantitative analysis of deadenylase enzyme activity.

Authors:  Maryati Maryati; Ishwinder Kaur; Gopal P Jadhav; Loyin Olotu-Umoren; Blessing Oveh; Lubna Hashmi; Peter M Fischer; G Sebastiaan Winkler
Journal:  Nucleic Acids Res       Date:  2013-10-28       Impact factor: 16.971

6.  Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).

Authors:  Tian-Li Duan; Han Jiao; Guang-Jun He; Yong-Bin Yan
Journal:  Cells       Date:  2020-01-09       Impact factor: 6.600

7.  Temperature-dependent fasciation mutants provide a link between mitochondrial RNA processing and lateral root morphogenesis.

Authors:  Kurataka Otsuka; Akihito Mamiya; Mineko Konishi; Mamoru Nozaki; Atsuko Kinoshita; Hiroaki Tamaki; Masaki Arita; Masato Saito; Kayoko Yamamoto; Takushi Hachiya; Ko Noguchi; Takashi Ueda; Yusuke Yagi; Takehito Kobayashi; Takahiro Nakamura; Yasushi Sato; Takashi Hirayama; Munetaka Sugiyama
Journal:  Elife       Date:  2021-01-14       Impact factor: 8.140

8.  Biochemical and in silico identification of the active site and the catalytic mechanism of the circadian deadenylase HESPERIN.

Authors:  Rafailia A A Beta; Athanasios Kyritsis; Veroniki Douka; Eirini Papanastasi; Marianna Rizouli; Demetres D Leonidas; Dimitrios Vlachakis; Nikolaos A A Balatsos
Journal:  FEBS Open Bio       Date:  2022-03-29       Impact factor: 2.693

9.  Biochemical and biophysical characterization of the deadenylase CrCaf1 from Chlamydomonas reinhardtii.

Authors:  Jia-Quan Zhang; Guang-Jun He; Yong-Bin Yan
Journal:  PLoS One       Date:  2013-07-23       Impact factor: 3.240
  9 in total

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