CONTEXT: Myostatin is a member of the TGF-beta superfamily and is primarily known for its ability to inhibit muscle growth. It also has actions on glucose metabolism. We hypothesized that it may act as a paracrine regulator of glucose uptake in the placenta, potentially contributing to fetal and placental growth. OBJECTIVES: The objective of this study was to determine whether myostatin is present in and formed by the human placenta and to evaluate its effects on glucose uptake. MATERIALS AND METHODS: Myostatin protein and mRNA were measured using Western immunoblotting and real-time PCR, respectively. Glucose uptake was assessed by uptake of radiolabeled deoxyglucose in vitro. Placental tissues were obtained at term (n = 8), preterm (n = 8; 24-34 wk), and early in pregnancy (n = 6; 9-13 wk). RESULTS: Human placentas were shown to express myostatin protein, with a significantly lower expression in term samples compared with samples collected in preterm samples. Human placentas express myostatin mRNA throughout gestation, which does not change. Myostatin treatment of human term placental explants resulted in an increase in deoxyglucose uptake compared with controls. CONCLUSIONS: Myostatin is synthesized, released, and acts within the human placenta. It contributes to placental glucose homeostasis and may be a therapeutic target in diseases ranging from placental insufficiency to diabetes in pregnancy.
CONTEXT: Myostatin is a member of the TGF-beta superfamily and is primarily known for its ability to inhibit muscle growth. It also has actions on glucose metabolism. We hypothesized that it may act as a paracrine regulator of glucose uptake in the placenta, potentially contributing to fetal and placental growth. OBJECTIVES: The objective of this study was to determine whether myostatin is present in and formed by the human placenta and to evaluate its effects on glucose uptake. MATERIALS AND METHODS:Myostatin protein and mRNA were measured using Western immunoblotting and real-time PCR, respectively. Glucose uptake was assessed by uptake of radiolabeled deoxyglucose in vitro. Placental tissues were obtained at term (n = 8), preterm (n = 8; 24-34 wk), and early in pregnancy (n = 6; 9-13 wk). RESULTS:Human placentas were shown to express myostatin protein, with a significantly lower expression in term samples compared with samples collected in preterm samples. Human placentas express myostatin mRNA throughout gestation, which does not change. Myostatin treatment of human term placental explants resulted in an increase in deoxyglucose uptake compared with controls. CONCLUSIONS:Myostatin is synthesized, released, and acts within the human placenta. It contributes to placental glucose homeostasis and may be a therapeutic target in diseases ranging from placental insufficiency to diabetes in pregnancy.
Authors: Arin K Oestreich; William M Kamp; Marcus G McCray; Stephanie M Carleton; Natalia Karasseva; Kristin L Lenz; Youngjae Jeong; Salah A Daghlas; Xiaomei Yao; Yong Wang; Ferris M Pfeiffer; Mark R Ellersieck; Laura C Schulz; Charlotte L Phillips Journal: Proc Natl Acad Sci U S A Date: 2016-11-07 Impact factor: 11.205
Authors: Chun Lung Wong; Ya Yu Huang; Wing Kei Ho; Hong Kit Poon; Pui Lai Cheung; Wai Sum O; Pak Ham Chow Journal: Reprod Biol Endocrinol Date: 2009-11-25 Impact factor: 5.211
Authors: Tingqing Guo; William Jou; Tatyana Chanturiya; Jennifer Portas; Oksana Gavrilova; Alexandra C McPherron Journal: PLoS One Date: 2009-03-19 Impact factor: 3.240
Authors: Ravi Thadhani; Camille E Powe; May Lee Tjoa; Eliyahu Khankin; Jun Ye; Jeffrey Ecker; Alan Schneyer; S Ananth Karumanchi Journal: Diabetes Care Date: 2009-12-10 Impact factor: 19.112