Literature DB >> 16461733

Freezing induces biased results in the molecular detection of Flavobacterium columnare.

Lotta-Riina Suomalainen1, Hilkka Reunanen, Ritva Ijäs, E Tellervo Valtonen, Marja Tiirola.   

Abstract

Specific PCR detection and electron microscopy of Flavobacterium columnare revealed the risk of false-negative results in molecular detection of this fish pathogen. Freezing and thawing destroyed the cells so that DNA was for the most part undetectable by PCR. The detection of bacteria was also weakened after prolonged enrichment cultivation of samples from infected fish.

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Year:  2006        PMID： 16461733      PMCID： PMC1392932          DOI： 10.1128/AEM.72.2.1702-1704.2006

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  Cytophaga columnaris (Davis) in Pure Culture: A Myxobacterium Pathogenic to Fish.

Authors:  L Garnjobst
Journal:  J Bacteriol       Date:  1945-02       Impact factor: 3.490

2.  Transmission and detection of Flavobacterium columnare in channel catfish Ictalurus punctatus.

Authors:  Thomas L Welker; Craig A Shoemaker; Covadonga R Arias; Phillip H Klesius
Journal:  Dis Aquat Organ       Date:  2005-02-28       Impact factor: 1.802

3.  Shieh medium supplemented with tobramycin for selective isolation of Flavobacterium columnare (Flexibacter columnaris) from diseased fish.

Authors:  A Decostere; F Haesebrouck; L A Devriese
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

4.  Rapid detection of columnaris disease in channel catfish (Ictalurus punctatus) with a new species-specific 16-S rRNA gene-based PCR primer for Flavobacterium columnare.

Authors:  Joel A Bader; Craig A Shoemaker; Phillip H Klesius
Journal:  J Microbiol Methods       Date:  2003-02       Impact factor: 2.363

5.  Comparison of first void urine and urogenital swab specimens for detection of Mycoplasma genitalium and Chlamydia trachomatis by polymerase chain reaction in patients attending a sexually transmitted disease clinic.

Authors:  Jørgen Skov Jensen; Eva Björnelius; Birthe Dohn; Peter Lidbrink
Journal:  Sex Transm Dis       Date:  2004-08       Impact factor: 2.830

6.  Diagnosis of flavobacteriosis by direct amplification of rRNA genes.

Authors:  Marja Tiirola; E Tellervo Valtonen; Päivi Rintamäki-Kinnunen; Markku S Kulomaa
Journal:  Dis Aquat Organ       Date:  2002-08-29       Impact factor: 1.802

7.  Optimization of PCR based detection of human papillomavirus DNA from urine specimens.

Authors:  Joeli A Brinkman; Meliha Z Rahmani; W Elizabeth Jones; Anil K Chaturvedi; Michael E Hagensee
Journal:  J Clin Virol       Date:  2004-04       Impact factor: 3.168
  7 in total
  3 in total

1.  Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals.

Authors:  Raju Sekar; Longin T Kaczmarsky; Laurie L Richardson
Journal:  Appl Environ Microbiol       Date:  2009-02-27       Impact factor: 4.792

2.  Differential effects of frozen storage on the molecular detection of bacterial taxa that inhabit the nasopharynx.

Authors:  Brenda A Kwambana; Nuredin I Mohammed; David Jeffries; Mike Barer; Richard A Adegbola; Martin Antonio
Journal:  BMC Clin Pathol       Date:  2011-01-24

Review 3.  Columnaris disease in fish: a review with emphasis on bacterium-host interactions.

Authors:  Annelies Maria Declercq; Freddy Haesebrouck; Wim Van den Broeck; Peter Bossier; Annemie Decostere
Journal:  Vet Res       Date:  2013-04-24       Impact factor: 3.683
  3 in total

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