Literature DB >> 16461726

A Bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium.

Marco Malten1, Rebekka Biedendieck, Martin Gamer, Ann-Christin Drews, Simon Stammen, Klaus Buchholz, Lubbert Dijkhuizen, Dieter Jahn.   

Abstract

A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed. Up to 1 mg/liter of a His6-tagged or Strep-tagged Lactobacillus reuteri levansucrase was directed into the growth medium, using the B. megaterium esterase LipA signal peptide, and recovered by one-step affinity chromatography.

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Year:  2006        PMID： 16461726      PMCID： PMC1392972          DOI： 10.1128/AEM.72.2.1677-1679.2006

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase.

Authors:  Cristian Ruiz; Ana Blanco; F I Javier Pastor; Pilar Diaz
Journal:  FEMS Microbiol Lett       Date:  2002-12-17       Impact factor: 2.742

2.  Site-directed mutagenesis study of the three catalytic residues of the fructosyltransferases of Lactobacillus reuteri 121.

Authors:  L K Ozimek; S A F T van Hijum; G A van Koningsveld; M J E C van Der Maarel; G H van Geel-Schutten; L Dijkhuizen
Journal:  FEBS Lett       Date:  2004-02-27       Impact factor: 4.124

3.  Coexpression of the type I signal peptidase gene sipM increases recombinant protein production and export in Bacillus megaterium MS941.

Authors:  Marco Malten; Hannes Nahrstedt; Friedhelm Meinhardt; Dieter Jahn
Journal:  Biotechnol Bioeng       Date:  2005-09-05       Impact factor: 4.530

4.  Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium.

Authors:  Marco Malten; Rajan Hollmann; Wolf-Dieter Deckwer; Dieter Jahn
Journal:  Biotechnol Bioeng       Date:  2005-01-20       Impact factor: 4.530

Review 5.  Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome.

Authors:  H Tjalsma; A Bolhuis; J D Jongbloed; S Bron; J M van Dijl
Journal:  Microbiol Mol Biol Rev       Date:  2000-09       Impact factor: 11.056

Review 6.  Prime time for Bacillus megaterium.

Authors:  P S Vary
Journal:  Microbiology       Date:  1994-05       Impact factor: 2.777

7.  Biochemical and molecular characterization of a levansucrase from Lactobacillus reuteri.

Authors:  S A F T van Hijum; E Szalowska; M J E C van der Maarel; L Dijkhuizen
Journal:  Microbiology       Date:  2004-03       Impact factor: 2.777
  7 in total
  15 in total

1.  High-yield intra- and extracellular protein production using Bacillus megaterium.

Authors:  Simon Stammen; Britta Katrin Müller; Claudia Korneli; Rebekka Biedendieck; Martin Gamer; Ezequiel Franco-Lara; Dieter Jahn
Journal:  Appl Environ Microbiol       Date:  2010-04-30       Impact factor: 4.792

2.  Genome sequences of the biotechnologically important Bacillus megaterium strains QM B1551 and DSM319.

Authors:  Mark Eppinger; Boyke Bunk; Mitrick A Johns; Janaka N Edirisinghe; Kirthi K Kutumbaka; Sara S K Koenig; Heather Huot Creasy; M J Rosovitz; David R Riley; Sean Daugherty; Madeleine Martin; Liam D H Elbourne; Ian Paulsen; Rebekka Biedendieck; Christopher Braun; Scott Grayburn; Sourabh Dhingra; Vitaliy Lukyanchuk; Barbara Ball; Riaz Ul-Qamar; Jürgen Seibel; Erhard Bremer; Dieter Jahn; Jacques Ravel; Patricia S Vary
Journal:  J Bacteriol       Date:  2011-06-24       Impact factor: 3.490

3.  A short story about a big magic bug.

Authors:  Boyke Bunk; Arne Schulz; Simon Stammen; Richard Münch; Martin J Warren; Manfred Rohde; Dieter Jahn; Rebekka Biedendieck
Journal:  Bioeng Bugs       Date:  2010-01-04

4.  Essential role of the glucosyltransferase activity in Clostridium difficile toxin-induced secretion of TNF-alpha by macrophages.

Authors:  Xingmin Sun; Xiangyun He; Saul Tzipori; Ralf Gerhard; Hanping Feng
Journal:  Microb Pathog       Date:  2009-03-24       Impact factor: 3.738

5.  Single cell analysis applied to antibody fragment production with Bacillus megaterium: development of advanced physiology and bioprocess state estimation tools.

Authors:  Florian David; Antje Berger; Robert Hänsch; Manfred Rohde; Ezequiel Franco-Lara
Journal:  Microb Cell Fact       Date:  2011-04-15       Impact factor: 5.328

6.  Comparative proteomic analysis of high cell density cultivations with two recombinant Bacillus megaterium strains for the production of a heterologous dextransucrase.

Authors:  Wei Wang; Rajan Hollmann; Wolf-Dieter Deckwer
Journal:  Proteome Sci       Date:  2006-10-05       Impact factor: 2.480

7.  Production of recombinant antibody fragments in Bacillus megaterium.

Authors:  Eva Jordan; Michael Hust; Andreas Roth; Rebekka Biedendieck; Thomas Schirrmann; Dieter Jahn; Stefan Dübel
Journal:  Microb Cell Fact       Date:  2007-01-15       Impact factor: 5.328

8.  High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium.

Authors:  Yang Yang; Rebekka Biedendieck; Wei Wang; Martin Gamer; Marco Malten; Dieter Jahn; Wolf-Dieter Deckwer
Journal:  Microb Cell Fact       Date:  2006-11-28       Impact factor: 5.328

9.  Metabolic engineering of cobalamin (vitamin B12) production in Bacillus megaterium.

Authors:  Rebekka Biedendieck; Marco Malten; Heiko Barg; Boyke Bunk; Jan-Henning Martens; Evelyne Deery; Helen Leech; Martin J Warren; Dieter Jahn
Journal:  Microb Biotechnol       Date:  2009-06-10       Impact factor: 5.813

10.  Expression of recombinant Clostridium difficile toxin A and B in Bacillus megaterium.

Authors:  Guilin Yang; Boping Zhou; Jufang Wang; Xiangyun He; Xingmin Sun; Weijia Nie; Saul Tzipori; Hanping Feng
Journal:  BMC Microbiol       Date:  2008-11-06       Impact factor: 3.605
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