Literature DB >> 12480114

Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase.

Cristian Ruiz1, Ana Blanco, F I Javier Pastor, Pilar Diaz.   

Abstract

The lipolytic system of Bacillus megaterium 370 was investigated, showing the existence of at least two secreted lipases and a cell-bound esterase. A gene coding for an extracellular lipase was isolated and cloned in Escherichia coli. The cloned enzyme displayed high activity on short to medium chain length (C(4)-C(8)) substrates, and poor activity on C(18) substrates. On the basis of amino acid sequence homology, the cloned lipase was classified into subfamily I.4 of bacterial lipases. Copyright 2002 Federation of European Microbiological Societies

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Year:  2002        PMID： 12480114     DOI： 10.1111/j.1574-6968.2002.tb11485.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  11 in total

1.  Production, secretion, and cell surface display of recombinant Sporosarcina ureae S-layer fusion proteins in Bacillus megaterium.

Authors:  Denise Knobloch; Kai Ostermann; Gerhard Rödel
Journal:  Appl Environ Microbiol       Date:  2011-11-18       Impact factor: 4.792

2.  Draft Genome Sequence of the Deep-Sea Bacterium Moritella sp. JT01 and Identification of Biotechnologically Relevant Genes.

Authors:  Robert Cardoso de Freitas; Estácio Jussie Odisi; Chiaki Kato; Marcus Adonai Castro da Silva; André Oliveira de Souza Lima
Journal:  Mar Biotechnol (NY)       Date:  2017-07-22       Impact factor: 3.619

3.  High-yield intra- and extracellular protein production using Bacillus megaterium.

Authors:  Simon Stammen; Britta Katrin Müller; Claudia Korneli; Rebekka Biedendieck; Martin Gamer; Ezequiel Franco-Lara; Dieter Jahn
Journal:  Appl Environ Microbiol       Date:  2010-04-30       Impact factor: 4.792

4.  Rhodococcus sp. strain CR-53 LipR, the first member of a new bacterial lipase family (family X) displaying an unusual Y-type oxyanion hole, similar to the Candida antarctica lipase clan.

Authors:  Arnau Bassegoda; F I Javier Pastor; Pilar Diaz
Journal:  Appl Environ Microbiol       Date:  2012-01-06       Impact factor: 4.792

5.  Helicobacter pylori EstV: identification, cloning, and characterization of the first lipase isolated from an epsilon-proteobacterium.

Authors:  Cristian Ruiz; Serena Falcocchio; F I Javier Pastor; Luciano Saso; Pilar Diaz
Journal:  Appl Environ Microbiol       Date:  2007-02-09       Impact factor: 4.792

6.  Engineering of Bacillus lipase by directed evolution for enhanced thermal stability: effect of isoleucine to threonine mutation at protein surface.

Authors:  Jyoti Khurana; Ranvir Singh; Jagdeep Kaur
Journal:  Mol Biol Rep       Date:  2010-02-03       Impact factor: 2.316

7.  A Bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium.

Authors:  Marco Malten; Rebekka Biedendieck; Martin Gamer; Ann-Christin Drews; Simon Stammen; Klaus Buchholz; Lubbert Dijkhuizen; Dieter Jahn
Journal:  Appl Environ Microbiol       Date:  2006-02       Impact factor: 4.792

8.  Expression and biochemical characterization of a thermophilic organic solvent-tolerant lipase from Bacillus sp. DR90.

Authors:  Ahmad Asoodeh; Shirin Emtenani; Shamsi Emtenani
Journal:  Protein J       Date:  2014-10       Impact factor: 2.371

9.  Bacillus sp. JR3 esterase LipJ: A new mesophilic enzyme showing traces of a thermophilic past.

Authors:  Judit Ribera; Mónica Estupiñán; Alba Fuentes; Amanda Fillat; Josefina Martínez; Pilar Diaz
Journal:  PLoS One       Date:  2017-07-25       Impact factor: 3.240

10.  Development of Versatile Vectors for Heterologous Expression in Bacillus.

Authors:  Øivind Larsen; Gro Elin Kjæreng Bjerga
Journal:  Microorganisms       Date:  2018-06-07
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